Suppose the following base sequence was found in a segment of one  strand of a DNA molecule: 3’ A-A-T-A-C-C-T-C-C-T-A-A-C-T 5’ What would be the bases in the complementary strand? Label the 3’ and the 5’ ends. Illustrate the DNA molecule below. Label the 3’ and the 5’ ends of both strands. Separate the above DNA molecule up to the seventh base. Add one primer for the leading strand complementary to the first base Adenine of the template strand. Add one primer for the lagging strand complementary to the seventh base Adenine of the template strand. Illustrate the DNA molecule. Label the 3’ and 5’ ends.

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Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna
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Problem 12QP: DNA contains many hydrogen bonds. Are hydrogen bonds stronger or weaker than covalent bonds? What...
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Suppose the following base sequence was found in a segment of one

 strand of a DNA molecule: 3’ A-A-T-A-C-C-T-C-C-T-A-A-C-T 5’

  1. What would be the bases in the complementary strand? Label the 3’ and the 5’ ends.
  2. Illustrate the DNA molecule below. Label the 3’ and the 5’ ends of both strands.
  3. Separate the above DNA molecule up to the seventh base. Add one primer for the leading strand complementary to the first base Adenine of the template strand. Add one primer for the lagging strand complementary to the seventh base Adenine of the template strand. Illustrate the DNA molecule. Label the 3’ and 5’ ends.
  4. Elongate the new strands up the seventh base by adding DNA bases complementary to the template strand. Illustrate the resulting DNA molecule. Label the 3’ and the 5’ ends of the template strands and the complementary strands.
  5. Elongate the new strands up the seventh base by adding DNA bases complementary to the template strand. Illustrate the resulting DNA molecule. Label the 3’ and the 5’ ends of the template strands and the complementary strands.
  6. Separate the DNA molecule up to the last base. Add one primer for the lagging strand complementary to the last base Adenine of the template strand. Illustrate the resulting DNA molecule. Label the 3’ and the 5’ ends of the template strands and the complementary strands.
  7. Remove the RNA primers from the leading and lagging strands and replace these with DNA bases. Illustrate the resulting DNA molecule. Use black color for the bases of the template/parental strands and green color for the bases of the complementary/daughter strands. Label the 3’ and the 5’ ends of all the strands.

 

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