Suppose you cultured yeast, that you had transformed with PMZ379 plus the AVT5 SGRNA, on a plate of YES agar plus salt ions. You then cultured untransformed yeast on a plate containing YES agar plus salt ions. Your results showed growth on both plates. Which of the following is the most reasonable interpretation of your results? Question options: A)The untransformed yeast spontaneously mutated to have a Čas9 gene that could silence the AVT5 gene. B)The yeast had a mutation in the AVT5 gene that made the protein non-functional. C)The sgRNÁ gene correctly guided the Cas9 protein to the AVT5 gene to silence it in the transformed yeast. D)The conditions for the experiment were so exceptional, that they promoted growth in both the modified and unmodified yeast.

Suppose you cultured yeast, that you had transformed with PMZ379 plus the AVT5 SGRNA, on a plate of YES agar plus salt ions. You then cultured untransformed yeast on a plate containing YES agar plus salt ions. Your results showed growth on both plates. Which of the following is the most reasonable interpretation of your results? Question options: A)The untransformed yeast spontaneously mutated to have a Čas9 gene that could silence the AVT5 gene. B)The yeast had a mutation in the AVT5 gene that made the protein non-functional. C)The sgRNÁ gene correctly guided the Cas9 protein to the AVT5 gene to silence it in the transformed yeast. D)The conditions for the experiment were so exceptional, that they promoted growth in both the modified and unmodified yeast.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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