Suppose you have prepared a mitochondrial extract that contains all the soluble enzymes of the matrix but has lost (by dialysis) all the low molecular weight cofactors. What must you add to the extract so that the preparation will oxidize acetyl-CoA to CO₂? Classify each cofactor based on whether it is required for the preparation to oxidize acetyl-CoA to CO₂. P₁ Required ADP (or GDP) TPP Answer Bank CoA-SH NAD Not required lipoic acid FAD

Suppose you have prepared a mitochondrial extract that contains all the soluble enzymes of the matrix but has lost (by dialysis) all the low molecular weight cofactors. What must you add to the extract so that the preparation will oxidize acetyl-CoA to CO₂? Classify each cofactor based on whether it is required for the preparation to oxidize acetyl-CoA to CO₂. P₁ Required ADP (or GDP) TPP Answer Bank CoA-SH NAD Not required lipoic acid FAD

Organic Chemistry

9th Edition

ISBN:9781305080485

Author:John E. McMurry

Publisher:John E. McMurry

Chapter29: The Organic Chemistry Of Metabolic Pathways

Section29.SE: Something Extra

Problem 51AP: In glycerol metabolism, the oxidation of sn-glycerol 3-phosphate to give dihydroxyacetone phosphate...

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