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- Serological testing:b. How would one identify the serovar of Escherichia coli O157:H7? What does the “O” and “H” stand for, respectively? Be sure to define any terms that you use in your response.Kligler’s Iron Agar:Q10) Give me the expected results for Pseudomonas aeroginosa, Salmonella typhimurium, and Escherichia coli. Pseudomonas aeroginosa, Salmonella typhimurium Escherichia coli.Order: Mycostatin 150,000 U stat availablity : 100,000 Units prepare a dose: using the label attached
- Tryptophan Hydrolysis GM-VE b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?TSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etcIf you unfortunately ingested five (5) E. coli cells O157:H7 at 9:00 AM, May 27, how many cells would be present by 11:00 PM, May 28. E. coli generation time is 20 minutes. (Disregard stomach acid effect)
- Chopped garlic often contains C. botulinum endospores and iscommonly sold submerged in olive oil, which creates an anaerobicenvironment. What is required to be added to garlic sold in thismanner?a. sodium chlorideb. citric acidc. waterd. A warning label describing the symptoms of botulism.t /g = (Log Nt – Log N0) /0.301 10 mL of a culture of Enterobacter at 104 cells/mL is introduced into 990 mL of the same broth at the same temperature (37° C) with adequate mixing. It is 8 p.m. the night before class on Tuesday at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before class?Identify which antibiotic was used per set-up (see figure). Describe the result in the graphs provided to help you explain your answer. Listed below are some essential information.Antibiotic A: 0.5 kDa protein, targets peptidoglycanAntibiotic B: 20 kDa protein, targets peptidoglycanAntibiotic C: Cationic antimicrobial peptideAntibiotic D: Targets lipopolysaccharideStaphylococcus aureus: gram-positive bacteriumVibrio cholera: gram-negative bacteriumMethanosarcina: an archaean bacteriumCationic antimicrobial peptides (CAMPs): these positively charged antibiotics are attracted to the negatively charged cell wall and membrane. They are hydrophobic, and they insert into the membranes to create pores.
- 3. You plate 3 different strains of the bacteria family Aspergillis on a MacConkey Agar plate. You notice that 2 have a yellow shade, 1 has a subtle pink color. Please interpret this information. Such as, the type of fermenters these bacteria are and what this says about their metabolism.An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.discuss the unique capacity of the Deinococci to tolerate desiccation and high dosses radiation