TSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etc
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TSA plates of microorganisms grown at 30°C or 37°C In words, describe the differences you see for each organism (E. coli M.,luteus ,S. marcescens and S. saprophyticus) grown at 30°C or 37°C (ie, amount of growth, color, etc
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- C. Why do we grow E.Coli at 37-celsius degrees? What is this in Fahrenheit? What else is roughly this temperature?"Microorganisms used a biological warfare." a. What is the scientific name of the microorganism/s and its general characteristics?b. Why those microorganisms are used in such process/product?c. How do/does the microorganism/s impact the lives of human?If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.
- in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45If you altered the conditions under which bacterial growth normally occurs (i.e. increase the temperature of incubating a culture from 35 to 40°C), what effect would this have on the bacterial growth curve of the organism under study?Antibiotic Code Zone of Inhibition (mm) S, I, or R? Penicillin P 15 Streptomycin S 8 Ampicillin A 25 Chloramphenicol C 14 Bacitracin B 5 QUESTIONS: Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain. 4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these…
- Describe the procedure for obtaining the minimuminhibitory concentration (MIC) for a chemical that isbactericidal for Escherichia coli. Contrast the action ofdisinfectants and antiseptics.Escherichia coli but not Pyrolobus fumarii will grow at 40°C,while P. fumarii but not E. coli will grow at 110°C. What ishappening (or not happening) to prevent growth of eachorganism at the nonpermissive temperature?Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?
- A. Observation of growth characteristics on selective and differential media Mannitol salt Agar Bacteria Growth (+/-) Describe growth characteristics Interpretation S. aureus E. coli P. aeruginosa M. luteusShow the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg?