Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Table 2. Optimum temperature determination for novel a-amylase Volume of pH 6.7 buffer (mL) Test Volume of 0.1% Volume of 0.9% Pre-incubation Absorbance Tube w/v starch (mL) NacI (mL) (°C) 1 3.50 1.00 0.50 30 0.276 3.50 1.00 0.50 40 0.320 3 3.50 1.00 0.50 50 0.422 4 3.50 1.00 0.50 60 0.288 3.50 1.00 0.50 70 0.124 3.50 1.00 0.50 80 0.195 B. One drop (negligible volume) of a relatively concentrated enzyme solution was added in each test tube. After ten minutes, the reactions were terminated and one drop of DNS reagent (negligible volume) was added. Absorbance were measured at the same wavelength above. What is the optimum temperature for the amylase enzyme? Justify your answer. LO

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter7: Statistical Data Treatment And Evaluation

Section: Chapter Questions

Problem 7.10QAP

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