Table A: Preparing 320 μL Glucose Standards Tube Label Approx. [Glucose] Standard (mg/mL) Std-1 0.000 Std-2 0.250 Std-3 0.500 Std-4 0.750 Std-5 1.000 Std-6 1.250 Volume of stock glucose if 2.00 mg/mL 0.000 με Volume of Milli-Q Water 320 με Actual Standard [Glucose] (mg/mL)
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Fill in the volumes required to make the standard glucose solutions of various conentrations provided. provide calculations as well.
![Table A: Preparing 320 μL Glucose Standards
Tube Label
Approx. [Glucose]
Standard (mg/mL)
Std-1
0.000
Std-2
0.250
Std-3
0.500
Std-4
0.750
Std-5
1.000
Std-6
1.250
Volume of stock
glucose if
2.00 mg/mL
0.000 με
Volume of
Milli-Q Water
320 με
Actual Standard
[Glucose]
(mg/mL)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1fabc3ca-e22c-4a82-92cc-264bbcbec85e%2F866be1cf-45ce-42ad-a39d-ee9c839458da%2F6j6u1qr_processed.png&w=3840&q=75)
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- Calculate KI' of the inhibitor from the information given. All information may not be needed to calculate. K'm = (29Ki+1.45x10^-10)/Ki Vmax = 11.7 µMs-1 Kcat = 130 s^-1 Vo = 3.0 μMs-1 S = 10 μM Et = 0.09 µM Inhibitor Concentration = 5x10^-12Convert the experimental glucose concentration provided in below from mM (millimolar) to g/100 mL. Glucose Concentration is in mM 1.927 mM. ● Take the 1/100 dilution factor into consideration ● M will open the doors for you to get to g. ● Once at M assume 1L of solution. (Hint: this will help you when converting from M to moles) ● You will also need to calculate the molar mass of glucose which has the formula C6H12O6A glucose solution is supplied in a repeated fed batch culture. The following parameters have been measured at t=2.5 hours; the system was under a quasi-steady state at this time. V = 1.0L, F = 0.22L/hr, S_0 = 98g/L, mu_m = 0.31/hr, K_s = 0.14 g-glucose/L, YM_X/S = 0.48 g-cells/g-glucose, Xt_0 = 33g (1) Calculate the value of V0 and S. (2) Calculate the value of Xt for t = 2.5hrs.
- Calculcate Kcat for PNP substrate for both enzyme concentrations. enzyme volume: 20 ul Bovine Intensince Alkaline phosphatase molecular weight: 140,000 Bovine intenstine Alkaline phosphatase activity: 300 units/ml and 14 units/mg extinction coefficient PNP: 18.5 abs (mM-1 cm-1) Vmax: 0.332 moles/sec a) enzyme 1 concentration: undiluted b) enzyme 2 concentration: 1:1 dilutionThe glucose content of samples can be determined using Nelson't test. The equation of the line for the standard curve preapred for varying concentrations of glucose is y = 3.0x + 0.2. Based from this information, determine the glucose concentration (in M) of a strawberry preserve having an absorbance reading of 1.35.1. pH cannot affect the enzyme - activity A. True B. False 2. It took 6 mins for the disappearance of blue-black color in test tube 4 with 60 degrees Celsius temperature. A. True B. False