TASK 1. From a 100-mL algal culture, 100-μL was mixed with 900-µL dilution buffer. From this dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer counting chamber. Following the same lab rules in the video, determine the percent cell viability if all 5 squares had the same count as the square below (show your solution): 2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your solution). 3. Research on what DAPI staining is and discuss its current applications in cell biology experiments.

TASK 1. From a 100-mL algal culture, 100-μL was mixed with 900-µL dilution buffer. From this dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer counting chamber. Following the same lab rules in the video, determine the percent cell viability if all 5 squares had the same count as the square below (show your solution): 2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your solution). 3. Research on what DAPI staining is and discuss its current applications in cell biology experiments.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…
a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.
Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).
Confluence cells added onto 100-wells plate, 2 ml trypsin added onto cells and 6 ml media added to dilute trypsin, 1 ml was used for counting, and average number of Cells counted was 24(DF 2) find out the the number of cells/ml and total number of cells in 7 ml? How many cells you need for 100 wells, assuming Number of cells/well is 10 to the power 4 cells?
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Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred
Sample Station 5 This is an MRVP broth. The reagents A and B for the VP test have been added. A. What observations can you make about this test? B. What interpretation can you make about this organism's ability to ferment glucose?
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For the juice samples in exercise 3-4, you will be using the pour plate technique. In this exercise, you will add juice to a petri dish and pour agar over the sample and mix. Why is the media (potato dextrose agar, PDA) kept in a water bath until you are ready to use it, and why must you work fairly quickly? (One response answers both questions). The media is already solid and will form chunks The media has a dye added to it that must be kept at 45°C until ready to use O PDA is a media that allows growth of many types of fungi, and the heat from the water bath keeps the fungal spores from growing. The media is kept liquid in the water bath with heat, removing the tube from the heat source will cause the PDA to solidify
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Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap please