Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. • Small = 1 cm x 1 cm x 1 cm • Medium = 2 cm x 2 cm x 2 cm Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the diagram on the next page. Record your data in Table 1.2. 7. Cleanup: HCl can be poured down the sink with water; agar cubes thrown in the trash. Cups should be rinsed and dried. Questions: 1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?
Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. • Small = 1 cm x 1 cm x 1 cm • Medium = 2 cm x 2 cm x 2 cm Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the diagram on the next page. Record your data in Table 1.2. 7. Cleanup: HCl can be poured down the sink with water; agar cubes thrown in the trash. Cups should be rinsed and dried. Questions: 1. What is the independent variable in the experiment? Why? 2. What is the dependent variable in the experiment? Why? 3. What possible variables should you be sure to hold Constant? Give three and explain why. 4. What is your control variable? Why is this a valid control?
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 24RQ
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Transcribed Image Text:Direction: Read and analyze the following laboratory experiment and answer the
following question.
PART 1: SURFACE AREA AND CELL SIZE
Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein
cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels.
Methodology:
1. Safety: Wear goggles and nitrile gloves while completing this lab.
2. Obtain three different size blocks of pink or blue agar. Using a ruler,
measure the length, width, and height of the three blocks given below. Cut
the agar according to the given dimension.
Small = 1 cm x 1 cm x 1 cm
Medium = 2 cm x 2 cm x 2 cm
•
• Large = 1 cm x 1 cm x 6 cm
3. Record your data.
4.
Pour HCl or vinegar into two small cups. Place the one larger "cell" into one
cup and the two smaller cells in the other cup. Start timing 30 minutes.
5. After 30 minutes, remove the cells and blot them dry with a paper towel.
6. Using your ruler, measure the distance the HCl has diffused into the blocks
as shown on the diagram on the next page. Record your data in Table 1.2.
7. Cleanup: HCl can be poured down the sink with water; agar cubes thrown
in the trash. Cups should be rinsed and dried.
Questions:
1. What is the independent variable in the experiment? Why?
2. What is the dependent variable in the experiment? Why?
3. What possible variables should you be sure to hold Constant? Give three and explain
why.
4. What is your control variable? Why is this a valid control?
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