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What happens if you dont' add maltose in the molten top agar when performing an plaque assay? What would be the expected results?
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- A phage-infected bacterial culture was subjected to a series ofdilutions, and a plaque assay was performed in each case, withthe following results. What conclusion can be drawn in the caseof each dilution? Dilution Factor Assay Results(a) 104 All bacteria lysed(b) 106 14 plaques(c) 108 0 plaquesHow will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? A. ~1.1 *10^6 PFU/mL B. ~1 *10^7 PFU/mL C. ~3 * 10^7 PFU/mL D. ~2 * 10^6 PFU/mL E. ~3.5 *10^6 PFU/mL F. ~1.1 * 10^5 PFU/mL G. ~3.5 *10^5 PFU/mL
- The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?If you had cultures of e cool and e aero genes and forgot to label them which of the following assays would you use to tell the two cultures apart?What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contamination
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