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- DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?generated after a cDNA library is made unique sequences in the genome useful for mapping using sequence information all of the aboveWhich of these is not a tool for comparing DNA sequences? BLAST Fasta PLINK A dotplot e.g. dotlet
- What does BLAST stand for? Basic Local Alignment Search Tool Basic Local Alignment SequenceTool Best Local Alignment Search Tool Best Local Algorithm Search Toolschematic diagram for the procedure in doing DNA extraction, isolation and quantificationPerform Progressive Alignment Method on the following 5 sequences and findthe best multiple sequence alignments.Sequence # 1: ATCCAATTTTSequence # 2: ACTGACCSequence # 3: ATGGCCATTSequence # 4: ATCTTCTTSequence # 5: ATTGCCATT
- How we prepared the sample of DNA and what kinds of tracking dye we add to sample in SDS-PAGE ? Please mention the source of the information. write the resources, any single resourceYou wish to align a 300 bp nucleotide sequence to a genome. Which of the following pairwise sequence alignment algorithms is BEST suited for this task? Global alignment or Local alignment? Please explain or elaborate why you chose this alignment algorithm.The following data shows bisulfite sequencing results for a small region of the genome. How many methylated C's are in the gene? Reference sequence: GAGACCTCG Bisulfite sequencing: GAGACCTTG
- Transcribe and translate the DNA strand Remember to use the start and stop sequences. ACGGTACCGTTAGCCGACATCGGGGACACTGACTCGDNA ladder is attached (the picture shows HindIII as H, EcoRI as E, BamHI as B). Now, determine the size of fragments created by EcoRI and BamHI. In the graph attached there is the fragment size of HindIII. HindIII EcoRI BamHI Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp) 23455 11985 7430 4375 2150 1850Which one of the following options would be a good way to identify the location of the poly A tail in the published DNA sequence for a gene? Look for AATAAA near the 3’ UTR of the DNA sequence. Look for TTTTTTTTTTTTTT… (many Ts) near the 3’ UTR of the DNA sequence. Look for AAAAAAAAAAA… (many As) near the 3’ UTR of the DNA sequence. Look for a stop codon.