How we prepared the sample of DNA and what kinds of tracking dye we add to sample in SDS-PAGE ? Please mention the source of the information. write the resources, any single resource

Q: The human genome is made from more than 3 billion nucleotides.  How many sites (loci) are…

A: According to the question,  The human genome is made from more than 3 billion nucleotides.  How many…

Q: a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y…

A: DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA…

Q: In order to determine the purity of a DNA sample. spectrophotometry can be carried out at…

A: DNA absorbs the UV light at an absorbance of 260nm of the wavelength.

Q: hat are the steps in creating a dna profile

A: DNA profiling is also known as DNA fingerprinting. It is often used to identify the origin and…

Q: 1000bg 800b 500bd 300bd 100bg Identify the sizes of the DNA bands in the agarose gel above using the…

A: Agarose gel electrophoresis is a separation technique mostly used for DNA molecules. Where a sample…

Q: Identify and match the possible cause(s) of the following unexpected results in agarose gel…

A: * Gel electrophoresis is an method used to separate mixtures of DNA and RNA, or proteins based on to…

Q: primer

A: PCR- it stands for the polymerase chain reaction. it is used to make many copies of a specific…

Q: Saenger’s DNA sequencing procedure. Include the method and the main reagents used

A: Its a DNA sequencing method

Q: Aside from Sanger Sequencing, what are the other DNA sequencing methods that are available today?…

A: Sanger sequencing is a DNA sequencing technology that uses electrophoresis and is based on DNA…

Q: DNA QUANTITATION Consult related references (textbooks or research articles) and list possible…

A: DNA QUANTITATION: Contaminants that can affect the purity of the DNA sample are as follows:

Q: when measuring the purity of a DNA sample? Please select the single answer that is most correct.

A: An instrument called spectrophotometer is able to measure the absorbance of specific wavelength of…

Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…

A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…

Q: ripe banana a better sample compared to an unripe banana for DNA extraction

A: Raw bananas are often preferred for DNA extraction because they have high number of cells per…

Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb

A: With the advancement of science and technology in the field of life sciences, there has emerged…

Q: Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how…

A: The answer to the first question is, 3 grams of agarose in 150 ml of TBE.   *to get the second…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: DNA is a hereditary molecule found in cells that transmits genetic information from one generation…

Q: Which of the following best describes the process of DNA seqencing.

A: DNA is mainly made up of the nucleotide and also contains genetic information in the genes. Every…

Q: Sequencing reactions are done in separate tubes for each ddNTP with a radioactive primer. Which…

A: In our Desire primer it  is 15 nucleotide long however on gels there are only 10 bands are present…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: Describe the characteristics of the extracted DNA, such as colour, shape, size, and consistency.

A: Deoxyribonucleic acid (DNA) is a genetic information-encoded molecule. This information is used to…

Q: Sanger DNA sequencing/ dideoxy sequencing was used as shown in the diagram below. The arrow…

A: Sanger's sequencing method. It is a method based on DNA synthesis. In normal DNA synthesis,…

Q: SANGER SEQUENCING NEXT GENERATION SEQUENCING  WRITE A PRECISE AND ACCURATE DIFFERENTIAL REPORT ON…

A: sanger sequencing and next-gen sequencing is that the sanger method only sequences a single DNA…

Q: Refer to the DNA profiles comparing the DNA obtained from the three suspects with the crime-scene…

A: The process of DNA Fingerprinting id best described as the process or technique which is used to…

Q: Similarities between DNA agarose gels and SDS-PAGE are:

A: Gel electrophoresis is the technique of separation of molecules of the basis of their size and…

Q: The following DNA fragment was sequenced by the Sanger method. The red asterisk indicates a…

A: DNA polymerase is an enzyme that catalyzes the synthesis of DNA molecules from nucleoside…

Q: Lane 1 Lane 2 Lane 3 Sample2: PBR3222 DNA Mass Base Pairs (ng/Sul) Sample1: TUC19 1988 :8888 4.000…

A: Agarose gel electrophoresis is a technique which is used to separate the DNA on the basis of their…

Q: Please check the pic. Find out where the divisions between fragments are , the number of fragments…

A: An important enzyme is EcoRI, plays an important role in recombinant DNA technology. These enzyme…

Q: Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the…

A: DNA is extracted from various sources to study the sequence of the DNAs from various sources and to…

Q: hich of the following ARE part of a typical PCR reaction mixture?     DNA ligase     dNTPs (mix…

A: Ans-  dNTPs(mix ofdeoxynucleoside tri-phosphates )

Q: DNA QUANTITATION Can you use the spectrophotometer to quantify proteins? Consult related references…

A: Quantitative methods are used to determine the quantity of a substance in a given sample. A…

Q: hematic diagram for the procedure in doing DNA extraction, isolation and quantification

A: The deoxyribonucleic acid isolation method frees deoxyribonucleic acid from the cell and {so}…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: A restriction enzyme, also known as a restriction endonuclease, is a bacterial protein that cleaves…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: PCR, including Real-Time PCR which is probably the most widely used molecular technique especially…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Gel electrophoresis is a gel technique that uses an applied electrical field to separate nucleic…

Q: Why does the temperature constantly fluctuate inside the PCR machine? What is the purpose of the…

A: PCR, or Polymerase Chain Reaction, is a biological technique invented by Kary Mullis.

Q: How many different loci does the FBI use for DNA profiling (fingerpriniting)?

A: Introduction: DNA fingerprinting is used to find the connection between criminal activity by…

Q: Extracting DNA From Strawberries What is the purpose of adding the extraction buffer (soap + salt)?…

A: Strawberries are octoploid that means they have 8 copies of each chromosome in the cell.…

Q: What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample…

A: DNA microarray is a collection of DNA sequences attached to a solid surface such as a glass slide…

Q: The table shows the presence/absence and size of DNA bands of known samples using gel…

A: Electrophoresis is the separation of molecules in an agarose gel, under the influence of an electric…

Q: The gel profile has plasmid DNA digested by different restriction enzymes in lanes 1 to 4 (left to…

A: First option is incorrect because in the given diagram of plasmid DNA gel profile the DNAs in lanes…

Q: DNA bands from each sample lane of the gel electropherogram below.

A: Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their…

Q: Identify three items (aside from water) that must be used in a Polymerase Chain Reaction (PCR) mi

A: Items that must be used in a Polymerase Chain Reaction (PCR) mixture are-   MgCl2. dNTPs. Taq…

Q: xplain how the spin columns were effective in collecting the isolated RNA from the sample and why…

A: The use of spin columns for RNA isolation is a common practice in molecular biology laboratories.…

Q: Wells Base pairs: 6650 4973 4.3cm 3148 2396 1585 8 Ocm 692 > 10 Acm DNA size Markers Vector-EcoRI…

A: Hi, Thanks For Your Question.  Answer : First, Let Us See The Ladder Size. Let's Do Rounding Off…

Q: What are the possible errors that could have occurred in the experiment of extracting the DNA.

A: Deoxyribonucleic acid (DNA) is a double-stranded molecule. DNA replication is the process by which…

Q: Which of the following criteria do you feel is the most important one to optimize for a DNA…

A: The technique of determining an individual's DNA traits is known as DNA profiling (also known as DNA…

Q: Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the…

A: The correct option is b 5'CCATTGG3'. Sanger sequencing ( method of DNA sequencing and it involves…

Q: Based on the quantitation data, please indicate how you would dilute the following samples. Fill in…

A: DNA shows maximum absorbance at the wavelength of 260 nm. Hence the concentration of DNA in solution…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.