The blob operon produces enzymes that convert compound A into compound B. The operon is controlled by regulator gene S. Normally, the enzymes are synthesized only in the absence of compound B. If gene S is mutated, the enzymes are synthesized in the presence and in the absence of compound B. Does gene S produce a regulator protein that exhibits positive or negative control? Is this operon inducible or repressible?

The blob operon produces enzymes that convert compound A into compound B. The operon is controlled by regulator gene S. Normally, the enzymes are synthesized only in the absence of compound B. If gene S is mutated, the enzymes are synthesized in the presence and in the absence of compound B. Does gene S produce a regulator protein that exhibits positive or negative control? Is this operon inducible or repressible?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
The lac operon produces enzymes that allow the bacteria E. coli to metabolize lactose, it is in an inducable operon. When lactose is present, will the repressor protein attach or leave the operon? How would this effect the production of lactose producing enzymes?
Operon X produces three enzymes that convert Compound A to Compound B. The R gene makes the regulatory molecule for Operon X. Normally, the three enzymes are only produced when Compound B is present. However, when the R gene is mutated (no R gene is made), none of the three enzymes are produced regardless of whether Compount B is absent or present. Is this opern inducible or repressible? Does the R gene produce an activator or repressor? Is the R protein made in an active or inactive form?
In the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the breakdown of the sugar __________ into monosaccharides. This enzyme can also hydrolyze the sugar analog ____________, which produces blue pigments following its breakdown. Since the lac operon is not always on the bacterial cells, we add the chemical ________ which prevents the lac depressor from binding to the operators, leading to constitutive expression of the operon.
If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a inducible, constitutive or repressible operon?
An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the DNA-binding domain of CAP and ablates the ability of CAP to bind to the CAP site. Under which condition will the genes of the lac operon be transcribed at high levels? Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose absent Glucose present, lactose present Under any of these conditions, the lac operon will not be transcribed at high levels
Which of the following describes the lac operon in E. coli when lactose and glucose is present in the culture medium? Select one: a. CAP inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. b. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes decreases. c. Catabolite activator protein (CAP) inactive and RNA polymerase does not completely bind to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases. d. CAP is active and RNA polymerase binds completely to the promoter of the lac operon and transcription of lactose-metabolizing enzymes increases.
A mutation at the operator prevents the regulator protein from binding. What effect will this mutation have in the following types of operons? a. Regulator protein is a repressor of a repressible operon. b. Regulator protein is a repressor of an inducible operon.
If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
. In the lac operon of E. coli, if glucose levels increase while lactose levels decrease, what will happen in the regulation of genes controlling the enzymes needed to catabolize lactose? the lac operon will be activated due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be activated due to CAP detachment from a site near the promoter the lac operon will be repressed due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be repressed due to CAP detachment from a site near the promoter all of the above
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+