What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

Q: Using a pipette, add 2 mL of suspended DEAE SEPHAROSE (anion) resin to a chromatographic column.…

A: DEAE Sepharose matrix is used in Anion exchange column. In the given method power of salt strength…

Q: What are some advantages and disadvantages of ionizing radiationas a method of control?

A: Sterilization involves physical methods for removing or controlling the microbes. The physical…

Q: what does chroomatography observation mean

A: Column fractionation was developed in the 1890s as a technique of separating the various components…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: what is the importance of getting retention factor (rf) value in chromotography tools?

A: Rf also known as retention factor is used in thin layer chromatography (TLC) and high performance…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…

A: This is the structure of Sulfamethoxazole.

Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…

A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…

Q: Why might Gamma irradiation be considered better than using UV radiation?

A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: The following strip of chromatography paper has been placed in a polar solvent Which pigments the…

A: In chromatographic techniques, including paper chromatography, the lighter molecules will travel the…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a…

A: A spectrophotometer is an instrument that is used to measure the intensity of light absorbed by the…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Complete the following table by calculating the total magnification of the different objectives of…

A: Microscope is used to visualize objects that are not visible to the naked eye. It consists of…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…

A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…

A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: If we wanted to measure the BSA absorbance without the Biuret reagent, which wavelength would we use…

A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…

Q: why steroids do not show luminescence on thin layer chromatographic plates?

A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…

A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…

Q: When using a light microscope, objective lenses can be found to have a magnification of all of the…

A: Light microscopes are devices that are used to magnify small objects by using visible light.…

Q: Describe some applications of ionizing and ultraviolet radiation.

A: APPLICATION OF IONIZING RADIATION it can be used in smoke detectors. It can be used in…

Q: What is the reason for the decrease in the ABS value over time when measuring in the…

A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…

Q: For microprojectile bombardment, the particles used are typically? a. Nickel b. Tungsten O c.…

A: Ttransformation is a biotechnological process where a foreign DNA molecule is inserted into the host…

Q: Based on the data chart below, how far did the solvent travel up the chromatography paper?

A:

Q: What is the importance of sllid width in fluorescence spectroscopy

A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.

Q: What is Ultraviolet (UV) light ?

A: The different kinds of EM radiation that are part of the electromagnetic spectrum, namely: Gamma…

Q: What major advantage does phase-contrast microscopy haveover staining?

A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…

Q: If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what…

A: Magnification is defined as a measure of the ability of a lens or other optical instruments to…

Q: What is the working principle of raman spectroscopy.

A: Raman spectroscopy helps in determining both qualitative and quantitative information of the analyte…

Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…

A: According to the question, the total magnification of a microscope is 700X.…

Q: Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a…

A: A compound microscope has two lens one is ocular or eyepiece lens and objective lens. Objective lens…

Q: Explain why is absorption used for studying the dyes, while fluorescence is used for studying the…

A: The investigation of various fundamental processes in the subject of life sciences relies on the…

Q: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

A: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

Q: Photoelectric effect increases the _________ in an x-ray image while Compton scattering increases…

A: X-ray is the procedure through which visualization of different body parts is done so that any…

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: Why can't we determine Vo, Ve and Vt for affinity chromatography?

A: Chromatographic techniques separate macromolecules based on size, ion exchange, or their affinity…

Q: why is it necessary to use oil immersion on oil objective ?

A: The oil immersion is the technique of light microscopy, which is used to increase the resolving…

Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…

A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…

Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…

A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…