In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
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In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
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- Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.how does the diameter of the column affect the flow rate of the elution in gel filtration chromatography?In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- Why does water separate out from a gel after freezing?In a gel filtration chromatography, what type of gel must be used when the protein size is 2500 Da? Explain.If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?a) High performance liquid chromatography) Thin layer chromatography) Adsorption chromatography) Gas-liquid chromatographyWhat will you do if you see bubbles forming on the gel after pouring it onto the gel caster/tray?
- The following strip of chromatography paper has been placed in a polar solvent Which pigments the most polar?briefly explain the principle behind this technique; gel-filtration chromatographyThere can be more than one correct answer Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatography