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The concentration of yeast RNA in an aqueous solution is 12.0 mg/L. The absorbance is found to be 0.208 when this solution is placed in a 1.00 cm cell at 265 nm. Its molecular weight is 5.7 x 103 g/mol. a)    Calculate the molar absorptivity of the yeast RNA.    b) What will be the absorbance if the solution is 6.0 mg/L and the path length is 1.00 cm? c)    What will be the absorbance if the solution is 12 mg/L and the path length is 2.00 cm?

The concentration of yeast RNA in an aqueous solution is 12.0 mg/L. The absorbance is found to be 0.208 when this solution is placed in a 1.00 cm cell at 265 nm. Its molecular weight is 5.7 x 103 g/mol. a)    Calculate the molar absorptivity of the yeast RNA.    b) What will be the absorbance if the solution is 6.0 mg/L and the path length is 1.00 cm? c)    What will be the absorbance if the solution is 12 mg/L and the path length is 2.00 cm?

Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.9: Spectrophotometry
Problem 4.14CYU
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The concentration of yeast RNA in an aqueous solution is 12.0 mg/L. The absorbance is found to be 0.208 when this solution is placed in a 1.00 cm cell at 265 nm. Its molecular weight is 5.7 x 103 g/mol.

a)    Calculate the molar absorptivity of the yeast RNA.   

b) What will be the absorbance if the solution is 6.0 mg/L and the path length is 1.00 cm?

c)    What will be the absorbance if the solution is 12 mg/L and the path length is 2.00 cm?

 

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