Determine the standard concentration of A through E and then the absorbance. I just need clarification on if I add together the two ml values given. Chem 116L; Fundamentals of Chemistry 1I Laboratory: Calibration Curves (Virtual Lab)4. Using Excel (or another graphing package of your preference) draw a 'scatter with smoothlines and markers' plot (see 'intro_excel section C) of absorbance (y-axis) vs. wavelength (x-axis) and estimate the maximum absorbance wavelength (the wavelength at which theabsorbance is a maximum). Appropriately format the graph and label the graph and axis('intro_excel' section D and section F)Maximum absorbance wavelength540nmNote that in the simulation it is assumed that the spectrophotometer has already beencalibrated with a blankPart 2. Create a calibration plotIn order to determine the concentration of an unknown solution, we first construct acalibration curve. This is done by creating five standard solutions of known concentrationand measuring the absorbance of each.In practice the standard solutions are made by pipetting a known amount of a stock solutionwith a certain amount of distilled water. Assuming the stock solution is 900. µM, determinethe concentrations of the standards A through E (give your values to three sig figs).Solnml stockml distilledstandard concabsorbancewater(µM).A.B.teanT old00 613.00C.17.00D.5.0021.00Show your calculations with working

Given: Concentration of stock solution = 900 µM And the solution's data given is,

In practice the standard solutions are made by pipetting a known amount of a stock solution with a certain amount of distilled water. Assuming the stock solution is 900. µM, determine the concentrations of the standards A through E (give your values to three sig figs). Soln ml stock ml distilled standard conc absorbance water (µM) A 5.00 5.00 5.00 9.00 5.00 13.00 5.00 17.00 5.00 21.00

In practice the standard solutions are made by pipetting a known amount of a stock solution with a certain amount of distilled water. Assuming the stock solution is 900. µM, determine the concentrations of the standards A through E (give your values to three sig figs). Soln ml stock ml distilled standard conc absorbance water (µM) A 5.00 5.00 5.00 9.00 5.00 13.00 5.00 17.00 5.00 21.00

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.23QAP

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