The concentration of glucose in an aqueous  solution can be determined by oxidation with ferricyanide ion, Fe(CN)63–.  The absorbance of the ferricyanide ion measured at 420 nm increases as the concentration of glucose increases. [Glucose], mg / mL Absorbance 0.005 0.132 0.010 0.290 0.020 0.498 0.030 0.642 0.040 0.750 Using the method of least-squares, give the equation of the best straight line through the points. Make sure that m=slope has 3 decimal places (XX.XXX) and y-intercept (b) has 2 significant figures (0.0XX). In your answer you should write in the form of y=mx+b. For instance, y = 12.316x + 0.072

Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.9: Spectrophotometry
Problem 4.14CYU
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The concentration of glucose in an aqueous  solution can be determined by oxidation with ferricyanide ion, Fe(CN)63–.  The absorbance of the ferricyanide ion measured at 420 nm increases as the concentration of glucose increases.

[Glucose], mg / mL

Absorbance

0.005

0.132

0.010

0.290

0.020

0.498

0.030

0.642

0.040

0.750

Using the method of least-squares, give the equation of the best straight line through the points. Make sure that m=slope has 3 decimal places (XX.XXX) and y-intercept (b) has 2 significant figures (0.0XX). In your answer you should write in the form of y=mx+b. For instance, y = 12.316x + 0.072

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