The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin (PAT), bisphenolA (BPA) and ochratoxin A (OTA) using a solid core Kinetex C18 column (150 mm x 4.6 mm i.d., 5 pm particle size) at a flow rate of 1.0 mL/min. Acidified water and methanol were used as the mobile phase under gradient-elution mode. Photodiode array detector and fluorescence detector were used for the detection of the separated solutes. HMF 0.44 РАT 0.03- H, но 0.01- 0.00 0.0 i.0 i.s 20 is 3.0 3.5 Time (min) OTA 18 HyG CH, 16 14- 12- HO 10 ВРА Time (min) (а) (1) Predict the order of polarity of the analyzed compounds. (ii) Explain your answers in (i).
The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin (PAT), bisphenolA (BPA) and ochratoxin A (OTA) using a solid core Kinetex C18 column (150 mm x 4.6 mm i.d., 5 pm particle size) at a flow rate of 1.0 mL/min. Acidified water and methanol were used as the mobile phase under gradient-elution mode. Photodiode array detector and fluorescence detector were used for the detection of the separated solutes. HMF 0.44 РАT 0.03- H, но 0.01- 0.00 0.0 i.0 i.s 20 is 3.0 3.5 Time (min) OTA 18 HyG CH, 16 14- 12- HO 10 ВРА Time (min) (а) (1) Predict the order of polarity of the analyzed compounds. (ii) Explain your answers in (i).
Chapter15: Electrophoresis And Other Separation Methods
Section: Chapter Questions
Problem 5P
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