The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin (PAT), bisphenolA (BPA) and ochratoxin A (OTA) using a solid core Kinetex C18 column (150 mm x 4.6 mm i.d., 5 pm particle size) at a flow rate of 1.0 mL/min. Acidified water and methanol were used as the mobile phase under gradient-elution mode. Photodiode array detector and fluorescence detector were used for the detection of the separated solutes. HMF 0.44 РАT 0.03- H, но 0.01- 0.00 0.0 i.0 i.s 20 is 3.0 3.5 Time (min) OTA 18 HyG CH, 16 14- 12- HO 10 ВРА Time (min) (а) (1) Predict the order of polarity of the analyzed compounds. (ii) Explain your answers in (i).

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Chapter15: Electrophoresis And Other Separation Methods
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The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin
(PAT), bisphenol A (BPA) and ochratoxin A (OTÁ) using a solid core Kinetex C18
column (150 mm x 4.6 mm i.d., 5 pm particle size) at a flow rate of 1.0 mL/min. Acidified
water and methanol were used as the mobile phase under gradient-elution mode.
Photodiode array detector and fluorescence detector were used for the detection of the
separated solutes.
HMF
0.04-
РАT
0.03
H.
но
0.02-
0.01-
0.00
1.0
i.s
Time (min)
0.0
0.3
2.5
3.0
3.5
20
18-
ОТА
HC CH,
16
14-
12-
HO
10-
HO,
HO,
ВРА
6.5
7.0
7.5
9.0
Time (min)
(a)
(1)
Predict the order of polarity of the analyzed compounds.
(ii)
Explain your answers in (i).
AU
Transcribed Image Text:The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin (PAT), bisphenol A (BPA) and ochratoxin A (OTÁ) using a solid core Kinetex C18 column (150 mm x 4.6 mm i.d., 5 pm particle size) at a flow rate of 1.0 mL/min. Acidified water and methanol were used as the mobile phase under gradient-elution mode. Photodiode array detector and fluorescence detector were used for the detection of the separated solutes. HMF 0.04- РАT 0.03 H. но 0.02- 0.01- 0.00 1.0 i.s Time (min) 0.0 0.3 2.5 3.0 3.5 20 18- ОТА HC CH, 16 14- 12- HO 10- HO, HO, ВРА 6.5 7.0 7.5 9.0 Time (min) (a) (1) Predict the order of polarity of the analyzed compounds. (ii) Explain your answers in (i). AU
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