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- Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.What did you notice about the Scalp Bald Human photomicrographs shown at (a) 40x vs (b) 100x TM? Why do you think your instructor is asking you to look at it at two different total magnifications?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceSuppose you conduct direct visible spectrophotometry with red fruit punch as your solution. Which wavelengths of light would you expect to be strongly absorbed? Which wavelengths are not strongly absorbed? Explain.Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer? Please explain in detail and clearly. Thank you!
- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?How is the UV spectrometer work? What is absorbance and how is it determined?Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?
- What color does an object appear if it absorbs light at both 420-500 nm and at 620-700 nm? a. Red b. Black c. Purple d. Green e. Deep blueConsider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?
