The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular mass ( M, ). A newly purified protein (X) run on the same gel showed a single band that had moved a distance of 55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm? Round off your answer to the nearest whole number. Protein M. Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 38.0 Glyceraldehyde-3- phosphate dehydrogenase carbonic anhydrase 36 000 29 000 50.0 Trypsinogen 24 000 54.0 Soyabean trypsin inhibitor 20 100 61.0 Myoglobin 17 800 69.0 Lysozyme 14 300 79.0 Cytochrome c 12 400 86.5

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter33: High-performance Liquid Chromatography
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The following table shows the distance moved in an SDS-polyacrylamide gel by a
series of marker proteins of known relative molecular mass ( M, ). A newly purified
protein (X) run on the same gel showed a single band that had moved a distance of
55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm?
Round off your answer to the nearest whole number.
Protein
M.
Distance moved (mm)
Transferrin
78 000
6.0
Bovine serum albumin
66 000
12.5
Ovalbumin
45 000
32.0
Glyceraldehyde-3-
phosphate dehydrogenase
carbonic anhydrase
36 000
38.0
29 000
50.0
Trypsinogen
24 000
54.0
Soyabean trypsin inhibitor
20 100
61.0
Myoglobin
17 800
69.0
Lysozyme
14 300
79.0
Cytochrome c
12 400
86.5
Transcribed Image Text:The following table shows the distance moved in an SDS-polyacrylamide gel by a series of marker proteins of known relative molecular mass ( M, ). A newly purified protein (X) run on the same gel showed a single band that had moved a distance of 55 mm. What is the M, of protein X if the tracking dye covered a distance of 95 mm? Round off your answer to the nearest whole number. Protein M. Distance moved (mm) Transferrin 78 000 6.0 Bovine serum albumin 66 000 12.5 Ovalbumin 45 000 32.0 Glyceraldehyde-3- phosphate dehydrogenase carbonic anhydrase 36 000 38.0 29 000 50.0 Trypsinogen 24 000 54.0 Soyabean trypsin inhibitor 20 100 61.0 Myoglobin 17 800 69.0 Lysozyme 14 300 79.0 Cytochrome c 12 400 86.5
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