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- Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography (d) Use three diagrams to illustrate the separation of three different proteins by thesemethods.A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange resin is suitable to use if the buffer has a pH of 7.0?a. A resin with a quaternary amino groupb. A resin with sulfonic acid groupc. A resin containing primary aromatic/aliphatic amino groupd. A resin that has carboxylic acid groupExplain how we would run a paper chromatography on an amino acid sample (explain the procedure or method). In a paper chromatography of an amino acid, the solvent front traveled 12.0 cm while the amino acid traveled 6.0 cm. What is Rf value of the amino acid?
- Briefly explain how cation exchange chromatography can used in protein separation?. An Analytical Chemist is analyzing a protein sample analysis using a light scattering technique. If the formulation of the subject sample has included surfactants or salt additives, which of the following would adequately apply? . The additives would impact the ionic strength of the sample B. The impact of additives influence can be nullified when using a Light Scattering technique. C. The effect of formulation additives would impact the protein sample analysis, and should be considered as a factor when analyzing the protein aggregation using light scattering technique.For the three tripeptides TED, RAY and ALI, determine in which order they will be eluted at pH 7.0 from cation exchange chromatography column.