how would you isolate the glycosylated insulin from the unglhcosylated forms? can you come up with a method to purify the protein (insulin with sugar group)? It can be a column or a combination of columns. (hint: use size exclusion chromatography or other method and affinity concept.)
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- Chemistry a. Are there methods other than spectrophotometry to determine protein concentration? If there is, please provide an explanation of this method along with its advantages and disadvantages! b. Explain the principle of protein separation by electrophoresis method! c. Can protein electrophoresis method be used to characterize proteins? Please explain why!Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography (d) Use three diagrams to illustrate the separation of three different proteins by thesemethods.
- 1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?You are assigned to separate a mixture of colourless amino acids present in protein molecule, using chromatography technique. You have a chrome paper (Filter paper sheet), TLC plate, chrome tank or beaker, Solvent (Hexane) and the mixture of amino acids. Answer the following questions related to your experiment. (a). Which of the chromatography technique (paper or thin layer) will give better separation in this experiment?Question: Can thin-layer chromatography (TLC) be used to differentiate between two closely related compounds with similar structures?
- 1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?As distillation method is used to seperate a homogenous mixture, which kind of mixtures chromatography can seperate? Give some example.If two samples have identical Rf values, in a Paper Chromatography of Amino Acids lab procedure, does this mean that they are necessarily identical molecules? Explain
- Chemistry 1. In anion exchange chromatography, the stationary phase negatively charged. a. True b. False 2. Give two (2) examples of a planar chromatographic technique:.Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Thank you for your help on these questions!What further testing should be done to extract and compare the composition of food dyes used In two types of candies.(except in paper chromatography)