The image below shows the Lineweaver-Burke plot for Enzyme catalysed reaction. Units for the Y- axis is (s/mmol) and X-axis is (1/mM). Use the data on the plot to calculate Vmax and Km for this reaction. Type your answers in the text box below clearly indicating which value is Vmax and which one is Km. You do not need to show your workings but don't forget your units. -0.4 Download -0.2 0.4 0.3 0.2 0.1 ..0.0. -0.1 0.0 ENZYME CATALYSED REACTION 0.2 0.4 y = 0.3086x + 0.0276 R² = 0.9967 0.6 0.8 1.0 1.2
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- Ali has measured the oxidative stability of palm oil sample under accelerated storage condition at 60*C for 6 days The following data were recorded for the peroxide value and p anisidine value of the palm oil after 5 days of storage time: Sample weight 1.0030 g Concentration of NazSzO3 = 0.001 N Volume of NazSzO3 titrated for sample solution = 1.35 ml Volume of NazSzO3 titrated for blank solution 0.20 ml Absorbance for sample solution = 0.907 Absorbance for blank solution 0.203 Calculate: i) peroxide value ii) p-anisidine value iii) TOTOX valueUse the equations of the lines plotted in regards to the data pictured to determine Km and Vmax in the absence and presence of the inhibitor. The Km is ____________mM and the apparent Km is ___________mM. The Vmax is ______________M/min and the apparent Vmax is ______________M/min. Therefore, this must be a _________________ inhibitor.An enzyme with Km value of 15.75 M and Vm is 30 micromole/L.min is used in a batch reactor. Calculate the time of reaction required for 88% substrate conversion where the initial substrate concentration was 160 M.