Name five functional traits of microorganisms. What dose the functional diversity describe? Do we analyze these traits with culture-dependent methods or culture-independent method?
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- What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?Why are complex media preferable to chemically defined media for routinecultivation of microorganisms?
- What is the relationship between dilution factors and the efficacy of antimicrobial agents in inhibiting bacterial growth?What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of pure cultures in thelaboratory?What is the advantage of using Molecular data compared to plated culture or biochemical techniques in identifying bacterial species, especially for oral microbes ?
- What is the description of the growth and growth pattern of the microorganisms in the spread plate method?Type of Growth: Surface or Sub-surface?What is the typical growth pattern of inocula?what agents will not be effective on this micro organism?Identify culture methods and conditions used for blood, wound,urine, fecal, and genital specimens. Of what importance areselective and differential growth media in pathogen detection,and what special conditions must be maintained for theisolation of anaerobic pathogens?
- What properties of the Snyder deep media allow us to detect susceptibility to dental caries?Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a “needle” used to inoculate?What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?