The lac operon regulates expression of genes required for the breakdown of lactose. Use the schematic diagram of the lac operon to help you answer the following questions about the lac operon. Representation of the Lac operon and upstream regions in E.coli (not drawn to scale!) Indicates 1,000s bases Indicates regions of DNA lacz lacz lacz gene gene gene 00000 0 Lac I promoter Lac I gene CRP lac Z lac lac Z lacY lacA binding site promoter operator gene gene gene When wild-type E. coli are grown in media with high lactose and no glucose, which of the following statements are true? There is a basal level of lac Z, Y and A transcription RNA polymerase can bind tightly to the lac Z promoter and activate a high level of transcription RNA polymerase can bind tightly to the lac I promoter and activate a high level of transcription CRP protein is bound to the CRP binding site
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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