The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] -

The measurement of LDH in the serum can be used to diagnose whether tissue damage has occurred. Students were tasked with the development of an assay for LDH activity in human serum samples from a control patient (healthy) and a patient who had suffered cardiac damage. Two samples were provided, labelled C and D (Control and Diseased serum). The students decided to follow the loss of absorbance of NADH in an appropriate assay buffer containing pyruvate as the substrate. Q1 (a) By examining the absorbance spectra of both NAD*and NADH below, comment why this approach is suitable strategy. [3] -

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter6: Oral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 7.2P

See similar textbooks

Similar questions

Angiotensin Converting Enzyme (ACE) inhibitors cause blood vessels to relax, thereby reducing blood pressure. Although Captopril is part of the group of antihypertensive drugs widely used as ACE inhibitors, new research is constantly being carried out with the aim of selecting compounds that are more kinetically advantageous than Captopril. Therefore, a BioPharma employee selected some prototypes and performed kinetic tests, finding the result shown in the figure below. Based on the statement and the graphic above, mark the incorrect alternative. A) It is a graph for determining kinetic parameters according to Lineweaver-Burk. In that case, the coefficient a B) Comparing the prototypes with the reference drug, Prototype 1 has a higher Vmax value. C) Comparing the prototypes with the reference drug, Prototype 2 has a lower Vmax value. D) Captopril has a Vmax of approximately 0.730 and Km of approximately 0.669. E) The two prototypes have Km values lower than those of Captopril and,…
what are the effects of PSTMB shown and why is it important to show effects of PSTMB in vitro effects of PSTMB on LDHA? https://www.nature.com/articles/s41598-019-40617-3
Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.
The enzymatic activity of PFK1 is generally measured by set- ting up a coupled enzyme assay system whereby aldolase, triose phos- phate isomerase, and glycerol-3-phosphate dehydrogenase are added to the assay mixture. For the latter enzyme, NADH is added and its change in concentration is readily monitored at 340 nm. Write the chain of reactions catalyzed by these enzymes using structural formulas, label substrates and products, and explain why the coupled en- zyme assay system leads to oxidation of NADH. While the chain of reac- tions is similar to those in glycolysis, there is a critical difference because of the dehydrogenase enzyme. Describe how this enzyme causes the chain of reactions to differ from those in glycolysis.
What is the change in concentration (umol/mL/min) for NADPH if the change in absolute absorbance per second from your DHFR functional assay analysis was 0.0035?
1.1)the following data duscribe an enzyme-catalyzed reaction(hydrolysis of cabobenzoxyglycyl-L-tryptophan) Plot these results using a lineweaver-Burk method, and determine values for Km and Vmax. substrate concenrate(mM) Velocity(mM.sec-1) 2,5 0.024 5 0.036 10 0.053 15 0.060 20 0.061 25 0.062
In blood sugar test strips, and enzyme which specifically converts b-D-glucose to D-gluconolactone and hydrogen peroxide. The H2O2 produces a color change which can then be read as total blood glucose. Since the enzyme is specific for the b anomer, how can the results be interpreted as total glucose?
The muscle enzyme lactate dehydrogenase catalyzes the reaction NADH and NAD+ are the reduced and oxidized forms, respectively, of the coenzyme NAD. Solutions of NADH, but not NAD+, absorb light at 340 nm. This property is used to determine the concentration of NADH in solution by measuring spectrophotometrically the amount of light absorbed at 340 nm by the solution. Explain how these properties of NADH can be used to design a quantitative assay for lactate dehydrogenase.
Which statement best describes the principle behind the succinate dehydrogenase (SDH) assay used in the assay of subcellular fractions from mung bean seedlings? Question 11 options: SDH oxidized succinate to fumarate, then fumarate reduced DCPIP. Succinate is blue in colour, which is eliminated by its oxidation to fumarate by SDH. SDH produced FADH2, then FADH2 reduced DCPIP. SDH generated DCPIP, then DCPIP oxidized succinate to fumarate.
The KM for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8 × 10−2 M, and the KM for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6 × 10−4 M. (a) Which substrate has the higher apparent affi nity for the enzyme? (b) Which substrate is likely to give a higher value for Vmax?
A scientist is studying the enzyme X which is an important point of regulation in the metabolism of the inhabitants of Sumeru. He developed four Akademiyan-derived compounds which may possibly work against this enzyme, and tested using an eudiometer the metabolic rate of the sample cell lines. Data are below. Time for each compound (min) Eudiometer volume reading (mL) A B C D 0 83.1 62.6 89.0 66.4 5 83.2 100.6 90.2 71.4 10 83.2 83.6 95.0 77.2 15 83.2 83.6 100 84.6 20 83.3 110.8 104.6 88.2 Show the properly labeled volume versus time plot for each eudiometer, with the equation of the line and R2. What is the most effective inhibitor among the four compounds? It was found that the most effective inhibitor exhibits uncompetitive inhibition. Illustrate the Lineweaver-Burk plots of uninhibited and inhibited enzyme X with properly labeled axes.
Hemoglobin is the oxygen carrier in the blood. What is the effect of each of the following treatments on the oxygen affinity of hemoglobin in vitro and how do these changes impact the T state vs. R state of the enzyme? A) Decrease in pH from 7.4 to 7.2. B) Increase in 2,3-BPG concentration.