Since the enzyme is specific for the b anomer, how can the results be interpreted as total glucose?
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In blood sugar test strips, and enzyme which specifically converts b-D-glucose to D-gluconolactone and hydrogen peroxide. The H2O2 produces a color change which can then be read as total blood glucose. Since the enzyme is specific for the b anomer, how can the results be interpreted as total glucose?
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- True or False. Do all reducing sugars produce a positive result in an Osazone test?In the estimation of glucose with benedict's reagent, what is resposnible for the white precipitate observed? Indicated the reaction responsible for this.Give one test to show the difference between reducing and non-reducing sugars. (Give the indication of positive test, such as color, precipitate formation, etc.) Is sucrose a reducing or non-reducing sugar? Explain. Is lactose a reducing non reducing sugar? Explain.
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?Does all reducing sugars give a positive result in the Osazone test?Instead of Molisch's test, what specific test can be used to detect the presence of pentose sugar?
- Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.In Fehling’s test, aldose are oxidized to aldonic acid. Kindly give the reaction and mechanism of Fehling’s test from glucose to gluconic acid and then to the positive result. What would the reaction be if the sample is a non-reducing sugar?The tests (fluorescent spot test, spectrophotometer assay, and cytochemical assay) use chemicals such as dicholorphenolindophenol or metosulfate 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. How do these measure the activity of G6PD?
- The lipase substrate emulsion contains 0.500 mg of olive oil per 3 mL Also, the molar mass of the olive oil is 885 g/mol. If all of the olive oil in a 3 mL reaction is "digested" by pancreatic lipase in a reaction, how many molecules of olive oil have been degraded?In the isolation of glycogen from liver, why it is necessary to filter the solution while it is still hot? Explain in 1-3 sentencesWhat are the results from these two tests? (catalase and bacitracin)