The molar extinction coefficients for A and B at 2=450 nm are ɛ=2,000. and ɛ=3000. M-1cm-1. At =650 nm, the molar extinction coefficients are ɛ=1,000. for both compounds. If a sample containing both species had absorbance values at 2=600 nm are A=0.070 and at 2=400 nm are A=0.185, what concentrations of A and B are present in the unknown solution? Assume that the absorbances are additive and that the measurements were made using a 1-cm cuvette. Show your work.
The molar extinction coefficients for A and B at 2=450 nm are ɛ=2,000. and ɛ=3000. M-1cm-1. At =650 nm, the molar extinction coefficients are ɛ=1,000. for both compounds. If a sample containing both species had absorbance values at 2=600 nm are A=0.070 and at 2=400 nm are A=0.185, what concentrations of A and B are present in the unknown solution? Assume that the absorbances are additive and that the measurements were made using a 1-cm cuvette. Show your work.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 14.9QAP
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