Beer's Law Plot for Tartrazine Concentration Absorbance Linear Fit for: Data Set | Absorbance (µM or umol/L) Abs = mx+b m (Slope): 0.02534 +/- 0.0003590 0.001 0.00 b (Y-Intercept): -0.0009147 +/- 0.008746 Correlation: 0.9997 0.252 9.94 RMSE: 0.01129 0.5 19.9 0.506 29.8 0.738 39.8 1.018 10 30 40 20 Concentration (uM) slope (m): 0.02534 1/(µM) y-intercept (b): -0.0009147 Absorbance
Beer's Law Plot for Tartrazine Concentration Absorbance Linear Fit for: Data Set | Absorbance (µM or umol/L) Abs = mx+b m (Slope): 0.02534 +/- 0.0003590 0.001 0.00 b (Y-Intercept): -0.0009147 +/- 0.008746 Correlation: 0.9997 0.252 9.94 RMSE: 0.01129 0.5 19.9 0.506 29.8 0.738 39.8 1.018 10 30 40 20 Concentration (uM) slope (m): 0.02534 1/(µM) y-intercept (b): -0.0009147 Absorbance
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 14.12QAP
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1) Calculate the molar extinction coefficient (molar absorptivity) of tartrazine in units of 1/(M·cm). Assume a cuvette path length of 1.00 cm.
2) If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?
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