Beer's Law Plot for Tartrazine Concentration Absorbance Linear Fit for: Data Set | Absorbance (µM or umol/L) Abs = mx+b m (Slope): 0.02534 +/- 0.0003590 0.001 0.00 b (Y-Intercept): -0.0009147 +/- 0.008746 Correlation: 0.9997 0.252 9.94 RMSE: 0.01129 0.5 19.9 0.506 29.8 0.738 39.8 1.018 10 30 40 20 Concentration (uM) slope (m): 0.02534 1/(µM) y-intercept (b): -0.0009147 Absorbance

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 14.12QAP
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1) Calculate the molar extinction coefficient (molar absorptivity) of tartrazine in units of 1/(M·cm). Assume a cuvette path length of 1.00 cm.

2) If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?

Beer's Law Plot for Tartrazine
Concentration
Absorbance
Linear Fit for: Data Set | Absorbance
(µM or umol/L)
Abs = mx+b
m (Slope): 0.02534 +/- 0.0003590
0.001
0.00
b (Y-Intercept): -0.0009147 +/- 0.008746
Correlation: 0.9997
0.252
9.94
RMSE: 0.01129
0.5
19.9
0.506
29.8
0.738
39.8
1.018
10
30
40
20
Concentration (uM)
slope (m): 0.02534 1/(µM)
y-intercept (b): -0.0009147
Absorbance
Transcribed Image Text:Beer's Law Plot for Tartrazine Concentration Absorbance Linear Fit for: Data Set | Absorbance (µM or umol/L) Abs = mx+b m (Slope): 0.02534 +/- 0.0003590 0.001 0.00 b (Y-Intercept): -0.0009147 +/- 0.008746 Correlation: 0.9997 0.252 9.94 RMSE: 0.01129 0.5 19.9 0.506 29.8 0.738 39.8 1.018 10 30 40 20 Concentration (uM) slope (m): 0.02534 1/(µM) y-intercept (b): -0.0009147 Absorbance
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