The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yieldsin the presence and absence of the quencher. φF/φF,0.

The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yieldsin the presence and absence of the quencher. φF/φF,0.

Inquiry into Physics

8th Edition

ISBN:9781337515863

Author:Ostdiek

Publisher:Ostdiek

Chapter10: Atomic Physics

Section: Chapter Questions

Problem 35Q

See similar textbooks

Related questions

Q: mple 3.1: When a copper surface is illuminated by the radiation of wavelength λ = 2537 A from a…

A: Disclaimer: “Since you have asked multiple question, we will solve the first question for you. If…

Q: What is the energy range (in joules and eV) of photonsin the visible spectrum, of wavelength 400 nm…

A: The energy of the photons with wavelengths at the ends of the visible spectrum are,…

Q: Why p-type is heavily doped in Solid state detector?

A: The solid state detectors have to be very sensitive towards the incident radiation. The P side in…

Q: dN/dE plot instead of N(E)

Q: Just how does a selenium photocopier function?

Q: Given that Eg =0.72 eV for a certain material, find the wavelength of peak solar response for the…

Q: Calculate the wavelength (in nm) for a photon with a frequency of 3.70e+15 Hz.?

Q: An HCl molecule vibrates with a natural frequency of 8.1 x1013 Hz What is the difference in energy…

A: Frequency, f = 8.1 x1013 Hz

Q: What minimum accelerating voltage is required to produce an x - ray with a wavelength of 70.0 pm?

A: The energy acquired by a photon when it is accelerated to potential difference ‘V’ is given by

Q: • The K-shell ionization energy of iron is 8500 eV, and its L-shellionization energy is 2125 eV.…

Q: A laser used to weld detached retinas puts out25-ms-long pulses of 640-nm light which average…

Q: A beam of 7.7-MeV a particles scatters from a gold foil of thickness o 0.32 μm. What fraction of the…

A: Given : The energy of α particles = 7.7Mev thickness of gold foil = 0.32μm

Q: What is the frequency (per second) and energy per quantum (in joules) of x-ray beams of wavelength…

A: Write the expression for the frequency of x-ray beams (Mo Ka).

Q: A simple Ge n-i-n photo-detector sample has a length of L= 30 µm and a hole lifetime of t, = 0.4 µs.…

Q: Qy whet is the expectal ian value of findwg aplatan of the photonicstete 147=3 IV> + H> in ver ticl…

A: |ψ>=13|V>+23|H> probability of finding photon in vertical polarization or in state |V>…

Q: Solve the following, if a television signal (video and audio) has a bandwidth of 4.5MHz, this signal…

A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…

Q: What minimum accelerating voltage would be required to produce an x-ray with a wavelength of 70.0…

Q: Show that in an atom, the photons in a 1240 nm infrared beam possesses energies of 1.00 eV.

A: Given data: Wavelength, λ=1240 nm=1240×10-9m Known data: Planck's constant, h=4.136×10-15eV/Hz Speed…

Q: What is the kinetic energy of an electron (in eV), if its de Broiglie wavelength is 8.08 Å? Answer:

A: Given: The de Broglie wavelength is 8.08 Ao.

Q: What minimum accelerating voltage would be required to produce an x-ray with a wavelength of 0.0280…

A: wavelength = 0.0280 nm = 0.28 Angstrom

Q: Show that the wavelength of a photon, measured in angstroms, can be found from its energy, measured…

Q: A detector has a responsivity of 0.12 ampere per watt at a wavelength of 1.06 μm. What is the…

A: Given data: The responsivity of the detector is Rd = 0.12 A/W. The wavelength of the detector is λ…

Q: What minimum accelerating voltage would be required to produce an x-ray with a wavelength of 0.0380…

Q: Fond kaferatire of photon Jass Link) after it has Underame a devemible adiabatic expanslon to 8…

A: Given, Initial temperature, T1 = 2K Initial Volume, V1 = V Final Volume ,V2 = 8V In adiabatic…

Q: Consider a helium atom (m= 6.6m10^-27 kg) that is confined to mobility along a line with a lenghth…

A: Given, He atom with mass m= 6.6×10-27kg Length, L = 12A0 = 12×10-10m…

Q: Find the minimum wavelength for x-ray with a) 35 kV and b) 45kV voltages.

A: Given data :- a) 35 kV b) 45 kV

Q: A NaI(Tl) detector is coupled to a photomultiplier tube having certain stages for electron…

Q: The diode laser is produced by (15 radiative recombination of electrons ?injected true O False

A: The diode laser is a semiconductor device in which the diode is pumped with the electric current…

Q: *Etample:1 An x-ry tabe is opunating at So co, a what is the marimum energy gained by elichrons? E…

Q: What is the binding energy in eV of electrons in magnesium, if the longest-wavelength photon that…

A: (given) Longest wavelength (λ) photon can eject electrons = 337 nm= 337×10-9m Kinetic energy of…

Q: An APD has a quantum efficiency of 50% at a wavelength of 500 nm in the absence of multiplication.…

Q: Assume we have a material with a work function of 4.87 eV. What is the maximum speed, in meteres per…

Q: Find the minimum wavelength for x- ray with a) 35 kV and b) 45kV voltages.

A: a) V =35kV =35000V The minimum wavelength, λmin=hceV where h is the Planck's constant =6.626×10-34…

Q: Threshold wavelength for a metal having work function W, is 2. What is the threshold wavelength for…

Q: 5.4 If the accelerating potential between the cathode and anode of Thomson's e/me apparatus is 182.2…

A: potential difference (V)=182.2 V FORMULA energy gained by electron when it passes through potential…

Q: Q2. An aluminum absorber of thickness 8 g/cm. The beam intensity is 8.24 J/ m s. a) Calculate the…

Q: An initially stationary electron is accelerated through a voltage 93.6 eV. What is it's de Broglie…

Concept explainers

Blackbody

Q.5 A blackbody radiates how much energy when the temperature of the body is elevated?

Question

The observed fluorescence lifetime in the absence of a quencher is 1.4 ns. In the presence of a quencher the fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the f luorescence quantum yields
in the presence and absence of the quencher. φ_F/φ_F,0.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, physics and related others by exploring similar questions and additional content below.

Similar questions

What is the energy range (in joules and eV) of photonsin the visible spectrum, of wavelength 400 nm to 750 nm?
• The K-shell ionization energy of iron is 8500 eV, and its L-shellionization energy is 2125 eV. What is the wavelength of Ka X-raysemitted by iron?
The mean lifetime of the excited state produced by irradiating mercury vapor with a pulse of 263.7 nm radiation is 2.5 x10- 8s. Calculate the approximate value for the width of the fluorescence line produced in this way.
An APD has a quantum efficiency of 50% at a wavelength of 500 nm in the absence of multiplication. If the device is operated with a reverse bias to give a multiplication factor of 8. Calculate: a. responsivity. b. the photocurrent of the APD if the incident optical power is 4 μW.
What is the half-value layer (HVL) for a material with attenuation coefficient of 0.7/cm?
For a given beam current and target thickness, why would you expect a tungsten target to produce a higher x-ray intensity than targets of molybdenum or chromium?
Find the minimum wavelength for x- ray with a) 35 kV and b) 45kV voltages.
In laser eye surgery, the laser emits a 1.45-ns pulse focused on a spot that is 32.5 mm in diameter. (a) If theenergy contained in the pulse is 2.95 mJ, what is the power persquare meter (the irradiance) associated with this beam? (b) Suppose a molecule with a diameter of 0.500 nm is irradiated by thelaser beam. How much energy does the molecule receive in onepulse from the laser? (The energy obtained in part (b) is more thanenough to dissociate a molecule.)
The HC1 molecule oscillates at a frequency of 87.0 THz. What is the difference (in eV) between its adjacent energy levels?
Experiments are performed with ultra-cold neutrons having velocities as small as 1.00 m/s. (a) What is the wavelength of such a neutron? (b) What is its kinetic energy in eV?
What is the wavelength of (a) a 12-keV X-ray photon; (b) a 2.O-MeV y -ray photon?
A carbon dioxide laser used in surgery emits infrared radiation with a wavelength of 10.6 µm . In 1.00 ms, this laser raised the temperature of 1.00 cm of flesh to and evaporated it. (a) How many photons were required? You may assume that flesh has the same heat of vaporization as water, (b) What was the minimum power output during the flash?