The porosity of the PAGE gel is determined by the ratio of acrylamide and the amount of (TEMED, bis-acrylamide, SDS, glycine, Coomassie blue, iodoacetate, acetate, His tag, ammonium persulfate). SDS
Q: n the living cell, free energy made from one reaction can be used to drive another in an energetical...
A: Glycolysis is also known as the Embden Meyerhof pathway and it is highly conserved from ...
Q: Select the two most hydrophobic amino acids. A. Leucine and Isoleucine OB. Arginine and Lysine OC Cy...
A:
Q: How do the standard amino acids differ from one another? O in whether or not the amino group is chir...
A: Peptides and proteins are made up of amino acids, which are molecules or building components. An ami...
Q: Choose the plot that best reflects the activity of an enzyme inhibited irreversibly. 100% A B C D ti...
A: Enzyme inhibitors are the substances that bind to enzyme either at active site or allosteric site so...
Q: Heteropolysaccharide structures are defined by: Question 23 options: Two or more different mon...
A: Carbohydrates are made up of carbon, hydrogen, and oxygen in the ratio of 1:2:1. The carbohydrates w...
Q: A two-step pathway that is activated by the secretion of glucagon and adrenaline
A: Glucagon and adrenalin, both induce an increase in hepatic glucose levels. However the mechanism of ...
Q: The melanocyte-stimulating hormone a-melanotropin has the following sequence: Ser-Tyr-Ser-Met-Glu-Hi...
A: Aminoacids are the building blocks of proteins that are linked by peptide bond. The sequence of amin...
Q: TRUE OR FALSE Palmitic oil is (soluble) to hot ethanol and chloroform.
A: Hi. Thank you for the question. The second question looks incomplete. Please provide the details abo...
Q: O His-Tyr Question 23 At low salt concentration, proteins are less soluble. Changing the solubility ...
A: Salting out : Protein precipitation at the high salt concentration
Q: One of the key biological characteristics of SARS-COV-2, as well as several other viruses, is the pr...
A: SARS-CoV-2 uses several members Protein as active antigens. One of the most important is the Spike p...
Q: Enzymes that catalyze DNA denaturation or melting are: gyrases topoisomerases helicases ...
A: DNA is a genetic material that is a double stranded structure polymer of nucleotides. DNA replicatio...
Q: A synthetic immunoglobulin (Protein X) was created by a scientist to recognize retroviruses. However...
A: SDS is a very common detergent used to denature and reduce protein. A protein X will not be function...
Q: Create a flowchart of Amino Acids and classify them depending on the polarity of its R group.
A: Proteins are known to play an important role in the growth and development of the body and are consi...
Q: Which of the following statements differentiates structurally albumin (a globular protein) structura...
A: Macromolecules are the higher molecular mass compounds. The macromolecules of living systems are ter...
Q: QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepar...
A: TAE buffer is made of Tris (strong base), acetate (acid), and EDTA (ethylenediaminetetraacetic acid)...
Q: Will a mutation from Distal histidine to phenylalanine shift the binding curve of hemoglobin?Will it...
A: Hemoglobin (Hb) is a iron containing metallo- protein molecule which carries oxygen from lungs to ti...
Q: Side chains of which two amino acids may participate in peptide bonding? A. Alanine and Glycine B. P...
A: -Glycine has H as side chain. -Alanine has CH3 as its side chain.
Q: 12.49.Explain why it is essential that fl uids used in intravenous injections have approximately the...
A: Intravenous fluids (IVFs) are one of the most used medical procedures for resembling physiologic bod...
Q: A ligand binds two different receptors with a Kd value of 10−7 M for receptor 1 and a Kd value of 10...
A: Introduction: A protein binds a ligand through a specific reversible reaction. The ligand-binding ca...
Q: What is the likely mechanism of spread of NDM-1?
A: NDM-1 is the protein product of a gene that some bacteria produce. A bacterial strain containing NDM...
Q: QUESTION 3, Out of starch, glycogen, cellulose and chitin, carbohydrate polymers with beta linkages ...
A: Introduction: Carbohydrates are aldehydes or ketones derivatives with multiple hydroxyl groups. It ...
Q: Analyzing: Analyze the following pair of monosaccharides and determine which of the following is an ...
A: Since there are multiple questions and they are not interlinked, as per our company guidelines only ...
Q: Which factor is least likely to result in protein denaturation? disruption of weak interactions by b...
A: Protein denaturation is the process by which a protein loses its tertiary structure and therefore it...
Q: What are the factors to be considered in designing an enzyme kinetics experiment??
A: Enzymes are biological catalysts that increase the rates of reactions in cell but do not themselves ...
Q: F1 progeny then mates with a short, brown, pretty male. The table below shows the res Based on the d...
A: To determine the correct order of the gene,we need to compare the recombinant frequencies among all ...
Q: Between glucose and fructose, which monosaccharide is metabolized faster? Why?
A: Both glucose and fructose are monosaccharides. Both undergo oxidation and produce two molecules of p...
Q: Give four enzymes that are being used in medical therapy. Report two industrial uses of enzymes and ...
A: Enzymes - Used for catalyzing various reactions for therapeutic , industrial uses
Q: 3. Below is the structure of a complex fatty acid that can be completely oxidized by the beta-oxidat...
A: Beta oxidation of fatty acid is catabolic process by which it converted to acetyl-CoA and Propionyl-...
Q: Glycosidic bond formation is what type of reaction? O Reduction O Hydrolysis O Oxidation
A: Monosaccharides are simplest sugar which form disaccharides, oligosaccharides and polysaccharides. ...
Q: The genetic data below is the DNA sequence of part of a theoretical fungal genome. a.) Which strand ...
A: DNA is a double stranded molecular structure with anti parallel stands of polarity 5' --->3' and ...
Q: Which statement about the composition of biological membranes is FALSE? Question 10 options: I...
A: The cell membrane is a lipid bilayer constituted of phospholipids and cholesterol. The membrane also...
Q: What is the meaning of proofreading activity ? O A. The polymerase checks for the correct incorporat...
A: DNA means deoxyribo nucleic acids. DNA act as genetic material in most organisms present on earth. T...
Q: Find a protein of your choice (rcsb.org), choose a part of it (containing at least 30 amino acid res...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids conta...
Q: 1H. please help me in detail. For molecular Mechanism of ATP versus GTP selectivity of adenylate kin...
A: ATP is the major energy carrier in any cell where as GTP is selective for only a few pathways. Adeny...
Q: What can be the interpretation for the results given below? Test/s Results Ninhydrin Xanthroproteic ...
A: Qualitative tests for amino acids help to detect the presence of specific amino acids and proteins i...
Q: (iv) Consider the molecule 1 being derivatized to yield molecule 2. How would you expect AG for the ...
A: Biomolecules are the molecules involved in several biochemical pathways and also part of important b...
Q: Find a protein of your choice, choose a part of it (containing at least 30 amino acid residues), fin...
A: A 60 amino acid long portion of the protein (an enzyme ) trypsin from a species of camel (Camelus dr...
Q: Give an alternative form of CpG methylation, given clear handwritten explaination!
A: DNA methylation is known to be one of the major epigenetic marks. The cytosines that are methylated ...
Q: need answer asap
A: Inhibitors are substances which have the capability to inhibit the enzyme activity by binding to the...
Q: H. Draw a plot showing reaction velocity as a function of substrate concentration for Ks = 50 µM, Vm...
A: As here given in the question, Vmax= 5uM S-1 Ks=50uM and Ki = 250uM both in presence and absence of ...
Q: Draw the orbital diagrams of the following elements: Silicon Охудen Chromium
A: Introduction: Orbital diagram help visualize which orbitals the electrons in an atom are. There are ...
Q: What is the term used to refer to the functional, folded conformation of proteins? Natural O Native ...
A: Proteins are the made up of several units of Aminoacids joined by peptide bond. There are 4 levels o...
Q: Cellulose is indigestible but necessary for O aiding in elimination O preventing stomach cancer O ho...
A: Cellulose a linear polysaccharide composed of glucose molecules linked via β-1,4 glycosidic bond. Hu...
Q: The porosity of the PAGE gel is determined by the ratio of acrylamide and the amount of (TEMED, bis-...
A: Introduction: Polyacrylamide gel electrophoresis is a subtype of gel electrophoresis whereby normal...
Q: Agarose Gel Electrphoresis of Serum Proteins Results 1 2 3 4 56 7 8 9 10 11 12 Wells Wells Chicken s...
A: Serum proteins are mainly Albumin and different classes (alpha, beta , gamma) of globulins. Albumins...
Q: Five amino acids were subjected to normal phase chromatography, and the following Rf values were com...
A: Retention factors are useful for the comparison of the results of chromatographic separation of one ...
Q: Which of the following about suicide substrates is incorrect? O They form a covalent bond with the a...
A: Suicide substrate are substrate molecule which remain inert until it binds to enzymes active site. O...
Q: Which among the following statements is correct? Group of answer choices Both statements are incor...
A: Chromatography is a technique used for separation of molecules based on the principle of distributio...
Q: Evaluating: Hydrogen bond is best represented as the electrostatic attraction between: * two hydroge...
A: 1. The hydrogen bond (H-bond) can be described as the electrostatic force of attraction between a hy...
Q: AZT (3'-azido-2',3'-dideoxythymidine) is a drug for HIV infection that gets incorporated into growin...
A: HIV is a retrovirus. Hence inorder for it to multiply inside the host , it first has to synthesize D...
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- please explain The porosity of the PAGE gel is determined by the ratio of acrylamide and the amount of .............................. (TEMD, bis-acrylamide, SDS, Glycine, Coomassie blue, iodocetate acetate, His tag, ammonium persulfate)A mixture of lipids is applied to a silica gel column (a column chromatography), and the column is then washed with increasingly non-polar solvents. The mixture consists of phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, cholesteryl palmitate (a sterol ester), sphingomyelin, palmitate, n-tetradecanol, triacyglycerol, and cholesterol. In what order will the lipids elute from the column? Explain the reasons for your answer(s).We have 50 X TAE (Tris-Acetate) stock in our laboratory and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).
- Given Tagatose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testGiven ß- Cyclodextrin Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testHow many mg of agarose do you need to weigh out to make 75 ml 1.3% gel?
- Given ß- Cyclodextrin, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a)Barfoed’s test (b)Seliwanoff’s test (c) Iodine’s testGiven Tagatose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a)Barfoed’s test (b)Seliwanoff’s test (c) Iodine’s testLactic acid 4 g Salicylic acid 5 g Trichloroacetic acid 2 g Flexible collodion qs ad 100 g Sig: wart remover. Use as directed (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. H ow many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v concentrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture? Answer: (a) 5.34 g camphor and 8.01 g castor oil (b) 8.39 mL castor oil (c) 3.12% w/v lactic acid, 3.91% w/v salicylic acid, and 1.56% w/v trichloroacetic acid Solution of the given answer is needed. Thank you so much and God bless. Happy new year
- Our laboratory has a stock of 50 X TAE (Tris-Acetate) and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).Given Raffinose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testGiven Sorbitol, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s test