the purpose of a mordant in Gram stain is    a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger  c. to fix iodine inside the cell d. to remove simple stain

Q: Which of the following statement is true? A. Gram negative bacteria have thicker…

A: Option (A) -  This is a incorrect statement.  Gram negative bacteria have a thinner peptidoglycan…

Q: How can natural pigments produced by bacteria reveal their Gram stain reaction? O Natural pigments…

A: Gram staining is a procedure through which a bacterial species can be classified and distinguishes…

Q: Which stain is most frequently used to distinguish differences between thecell walls of medically…

A: The cell walls of bacteria are made up of peptidoglycan- a layer of polysaccharide chains cross…

Q: A student was observing a filament of blue green bacteria under low power (60X). The diameter of the…

A: A. Average length of one cell = 2.4 mm/ 40=0.06 mm Under 60X = 40 cells, each is 0.06 mm in length

Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…

A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…

Q: 3. Draw the specimen observed under endospore staining  4. Draw the specimen observed under…

A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…

Q: The presence of a capsule around bacterial cells usually indi-cates their increased disease-causing…

A: They are stained using dyes. The dyes can be cationic or anionic. Simple, differential and…

Q: charge of a basic dye adheres to the charge of bacterial cell surfaces. 3. The a. micrometer b.…

A: Basic dyes are water-soluble cationic dyes used to impart colour. On the other hand bacteria is a…

Q: For a successful direct staining, one should A. use a minimal amount of water to speed up the…

A: Direct staining is the process to make slides using a single stain or dye.

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…

A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…

Q: Dyes are used to stain specimens that will be observed using the microscope to   a.  increase the…

A: Ans (d) : Dyes are used to stain specimens that will be observed using the microscope to make it…

Q: Give at least one disease that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore…

A: Staining is a microscopic technique for enhancing contrast in the material. In histology, as well as…

Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…

A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…

Q: b. Successfully completed most steps of the Gram stain but failed to add the iodine (the mordant):…

A: Gram staining is a differential staining procedure used to classify two types of bacteria on the…

Q: What is the decolorizer for the spore stain?

A: Spore Staining is a technique used in microbiology to differentiate vegetative cells and…

Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…

A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…

Q: a) What is Gram staining?     b) Describe step by step the procedure     c) Explain the basis of…

A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: The Gram Stain is able to stain bacterial cells differently depending upon the structure of their…

A: Bacterial cell wall is made up of peptidoglycan. it prevents the cell from bursting when it absorbs…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: A differential stain can: separate bacteria into groups by cytoplasm composition OA. separate…

A: The small parts of the microorganisms are hard to differentiate as the colors under the microscope…

Q: Imagine you are using a microscope with 5x eye pieces (instead of 10x like ours). If you change from…

A: given that the initial two magnifications are 5x and 10x , and final magnification is 5x and 40x

Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…

Q: If the stain is too red to the naked eye, what can be done to correct this? O that is normal O check…

A: Staining is a microscopy auxiliary method used to improve contrast in the microscopic image. Stains…

Q: Bacterial smears are fixed before staining to make their walls permeable. accept stain, O affix the…

A: By staining microbial smears on a microscope slide, bacteria and bacterial components such as…

Q: Bacteria may be Gram + or Gram - due to their difference in staining. This stain is significant to…

A: Gram staining is one of the most common methods of staining used to classify bacterial species into…

Q: Following a gram stain, you observe clear, glassy areas inside the bacterial cells. What would you…

A: Gram staining is a technique that is used to differentiate between bacterial cells on the basis of…

Q: Attached below is the study about the effects of handwashing and the effectiveness of soap against…

A: We only become aware of the significance of handwashing during pandemics. However, its advantages…

Q: Explain the purpose of this stain. Give an example of a type of clinically relevant bacteria that…

A: It is true that endospores appear as clear spots within cells under a light microscope following…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to…

A: The staining procedure is a multi-step process that involves the smear preparation followed by…

Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: 4. The Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the…

A: Decoloriser in gram staining method is to dehydrate the peptidoglycan layer of the cell wall causing…

Q: disease (each) that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore stain d.…

A: Since you have posted a question with multi- sub parts , we will solve first three sub-parts for…

Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…

A: Microscopy is the technique which is used to magnify  small microorganisms and small specimens . It…

Q: In order to have a better visualization of the shape acid the fast stain, you should do the…

A: Use of carbol fuchsin on the bacteria and heating it p, makes the cell appears red, presence of…

Q: You have a microscope with a resolution of 0.5 um. Your bacteria on your sample are 0.3 um apart on…

A: The light microscope is a versatile instrument used to study a wide variety of types of specimens…

Q: Microscopic samples can be stained. the stains are used to increase and improve O resolution and…

A: Staining It is defined as a technique through which the cells or thin sections of biological tissue…

Q: The Gram stain works because of differences in the O 1) cell membranes O 2) genetic characteristics…

A: Answer 1 cell membrane

Q: If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the…

A: Bacterial staining is a very important process to visualize the bacterial cell morphology. If there…

Q: You partner stained bacterial cells and saw only the background and not the actual cell was stained.…

A: Staining technique is a method in which recognition and visualisation of structure ,  arrangement of…

Q: Explain how you would troubleshoot a Gram stain of a known G(-) organism that stained purple.

A: Gram staining is used to differentiate bacteria based on their cell wall constituents. Gram-positive…

Q: Why might gram positive bacteria incorrectly appear to be gram negative after the gram staining…

A: Gram staining is a procedure which is used to identify whether the bacteria being tested in gram…

Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine  C.…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…

A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…

Q: The resolving power of the microscope reveals: a. Whether or not oil is needed to see a…

A: The instrument that is utilized in the laboratories to visualize the objects or structures that are…

Q: What is the difference between gram positive and gram negative bacteria? Describe how we use this to…

A: The following are the important differences between gram-positive and gram-negative bacteria:…

Q: A) The equation "d = A/(2 NA)" describes the of a lens system B) In the flagella stain, the mordant…

A: Ans 1:- The given equation describes the Abbe's Equation which summarises the resolution for a lens…

Q: The gram stain is a ______________ stain in which a decolorization step occurs between the…

A: Gram stain is a popular microbiological staining technique for identifying and characterising…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.