Q: The process that most accounts for magnification is a. a condenser c. illumination b. refraction of…
A: Magnification means the image produced is more than the size of the object.
Q: What is the importance of Gram-staining?
A: Introduction : Gram Staining, Commonly Known As Gram's Method, Is A Staining Technique That Is Used…
Q: Explain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram…
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: Which stain is most frequently used to distinguish differences between thecell walls of medically…
A: The cell walls of bacteria are made up of peptidoglycan- a layer of polysaccharide chains cross…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…
A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…
Q: hich Is The More Important Step In Gram Stain?
A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
Q: Explain the function of water in spore staining.
A: Spores are microscopic units that are accountable for asexual reproduction. Several species of…
Q: What might happen if the Gram staining procedure is performed ona culture incubated for a little…
A: Gram staining is one of the staining methods used in laboratories to differentiate between two broad…
Q: Give two reasons for the differential staining between gram + and gram - organisms
A: Differential Staining is all those staining methods that employ more than one stain to distinguish…
Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…
A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…
Q: Describe what we see in the Figure below, what do the colours indicate/what could they stain for?…
A: By the use of immunohistochemistry (IHC) we can visualise the different components of a cell by…
Q: What is the color of endospores after Gram staining? After spore staining?
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: What is the importance of Gram-staining
A: Gram staining was created by Christian gram Bacteria with Peptidoglycan in their cell walls are…
Q: What features of your Gram stain exemplify the Gram stain theory?
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: What is meant by Gram positive? Explain the mechanism of Gram positive staining.
A: Microorganisms or microbes are tangible objects that appear under a microscope. Large classes of…
Q: -What are the steps of gram staining -Which of the four steps is the most important and why? - What…
A: Based on the chemical and physical characteristics of their cell walls, the Gram stain is an…
Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…
A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…
Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…
A: The microorganisms that can be found in several areas of the habitat and come under the category of…
Q: 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast…
A: Microorganisms are microscopic organisms that are not visible to naked eyes. Several microorganisms…
Q: Outline the steps in gram staining
A: The common microbial technique that is used to differentiate the two large groups of bacteria…
Q: what is gram staining
A: Bacteria is a vast group of microorganisms that consists of various shapes, sizes, structure and…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…
A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: Match the following regarding the Gram stain.
A: Depending upon the structure of cell wall, bacteria are divided into two major classes. The basis of…
Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Discuss the various theories on the Gram stain.
A: Bacteria can be classified into two major types according to gram staining: Gram positive. Gram…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: The Gram stain is the most generally utilized staining methodology in bacteriology. This is an…
Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: You use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the…
A: Gram staining is a special technique in which bacteria are stained with gram stain. It is used to…
Q: Give two factors that may affect a good gram staining technique output. Provide an explanation how…
A: Many factors can affect the results of gram staining. However, two important factors that may affect…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Explain how you would troubleshoot a Gram stain of a known G(-) organism that stained purple.
A: Gram staining is used to differentiate bacteria based on their cell wall constituents. Gram-positive…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: What type of stain is the Gram stain and what does it rely on for meaningful results
A:
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: What is acid fast staining and why do we use gram staining more?
A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…
Q: Briefly describe the Gram stain
A: The Gram staining is a special technique in which bacteria are stained with the Gram stain. It is…
Q: in gram staining: what had happen when the following were applied: Dye, Mordant, Decoloriser,…
A: The bacteria are classified into two categories depending on the nature of cell wall. they are…
Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…
A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be found everywhere. They…
Q: What is the relationship between Gram staining and cell wall composition
A: Gram-positive bacteria have dense layers of peptidoglycan in their cell walls. Gram-negative…
Q: What is the difference between gram positive and gram negative bacteria? Describe how we use this to…
A: The following are the important differences between gram-positive and gram-negative bacteria:…
What are the expected result after
a. Crystal Violet =
b. Gram Iodine =
c. Ethanol =
d. Safranin =
Step by step
Solved in 2 steps
- Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few secondsExplain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram staining.Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)
- Give the principle of acid fast staining and gram staining.the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stainWhich of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C. Ethanol D. Crystal Violet
- Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingIf you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstainedWhat would you observe if you decolorize your slide too much for gram staining?
- a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.what effect would failing to drain the smear in-between staining have on your judgment of the gram reaction of an organism?Explain the principle of Gram staining. (You do not need to describe the procedure step by step).