If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected.

If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.
You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors have occurred?
Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
If you gram stain an acid fast organism, what would you most likely see when you examine the stain under oil immersion? You are in a hurry to test a bacterial culture for spore production. You grow the culture or 12 hours and then stain it. Your results are negative. Should you trust your results? Why or why not?
You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorless
You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.
Which of the following statements is FALSE? Question options: stains increase the contrast of a specimen with its surroundings all of these statements are TRUE dyes generally consist of a chromophore and an ion the chromophore of an acidic dye is positively-charged simple staining procedures use a single dye basic dyes bind to negatively-charged molecules
You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purple
An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not used
Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.
You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you found that the oil present in the lab was labeled as “vegetable oil”. Do you think it will be possible to use this oil to observe bacteria clearly? Explain your answer