Q: c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain.…
A: Stain is a compound or dye which is used to impart colour for the bacterial cell surface so that we…
Q: In the light microscope, which part holds the specimen 16 15 14 13 12 11 10 Select one: a. 13 O b.…
A: It is an instrument for visualizing fine detail of an object. It does this by creating a magnified…
Q: Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0…
A: Please follow step 2 & 3 for detailed explanation.
Q: The presence of a capsule around bacterial cells usually indi-cates their increased disease-causing…
A: They are stained using dyes. The dyes can be cationic or anionic. Simple, differential and…
Q: For a successful direct staining, one should A. use a minimal amount of water to speed up the…
A: Direct staining is the process to make slides using a single stain or dye.
Q: The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the…
A: Here we have to explain about why sediment observed in stained blood smear.
Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…
A: Introduction Capsule is the outer most covering, which is present even outside the cell wall. This…
Q: Give at least one disease that can be diagnosed by: a. Gram Stain b. Acid-fast stain c. Endospore…
A: Staining is a microscopic technique for enhancing contrast in the material. In histology, as well as…
Q: A cell is 25 μm wide when viewed at 1,000× magnification. Thismeasurement can also be written…
A: 1,000× magnification means that to the naked eye, the image would appear 1000 times larger than it…
Q: 8- The following are methods of sterilization EXCEPT: O c) Sterilization by filters O b) Autoclaving…
A: Introduction: A combination of heat, chemicals, irradiation, high pressure, and filtration can be…
Q: Using a light microscope, samples can be viewed by which method (choose all that apply) O dissecting…
A: An optical microscope is another name for a light microscope.
Q: 2. Explain the relationship between bacterial cell wall with gram positive and gram positive in the…
A: Gram staining is the procedure that is used to differentiate between the gram positive and gram…
Q: What is the decolorizer for the spore stain?
A: Spore Staining is a technique used in microbiology to differentiate vegetative cells and…
Q: Which stain is used 1st after you heat fix the fly during the gram staining procedure
A: Gram staining is the procedure by which the gram positive and gram negative bacteria can be…
Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…
A: The micro organisms are grown in specific containments for identification of the characteristic…
Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…
A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…
Q: A differential stain can: separate bacteria into groups by cytoplasm composition OA. separate…
A: The small parts of the microorganisms are hard to differentiate as the colors under the microscope…
Q: 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast…
A: Microorganisms are microscopic organisms that are not visible to naked eyes. Several microorganisms…
Q: Motility is best observed with a a. hanging drop preparation c. streak plate b. negative stain d.…
A: Motility is the ability of bacterial cells to migrate from one location to another. The flagella…
Q: 1. Define Differential Staining. 2. Application of differential staining technique.
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: If the stain is too red to the naked eye, what can be done to correct this? O that is normal O check…
A: Staining is a microscopy auxiliary method used to improve contrast in the microscopic image. Stains…
Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…
A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…
Q: the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell…
A: A mordant or color fixative is defined as a substance used to set (for example tie) colors on…
Q: The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin…
A: Electron microscope: It is a microscope which obtains a high-resolution image of non-biological as…
Q: 4. The Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the…
A: Decoloriser in gram staining method is to dehydrate the peptidoglycan layer of the cell wall causing…
Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…
A: Microscopy is the technique which is used to magnify small microorganisms and small specimens . It…
Q: J Gram Stain 1 A capsule Stain
A: Specimen is withdrawing blood, collecting urine, or swabs from mucosal surfaces.
Q: 13. A staining that divides organisms into two groups depending on their staining is referred to as…
A: Staining is a technique used for enhancement of contrast in samples mostly at microscopic…
Q: Write out the steps for this specfic stain. List them in the correct order
A: Organisms can be tentatively recognized via developing them on media and searching at morphology or…
Q: Gram Stain is used to differentiate. O 1. Virus O 2. Onion Cells O 3. Blood Cells O 4. Bacteria
A: Microbial organisms occur below micron levels in their size. So to distinguish them properly we have…
Q: 7. Draw what you would expect to see for acid fast stain and endospore stain procedure if you did…
A: Staining is a technique for adding color to cells, tissues, or microscopic components so that they…
Q: The main of objective of using 13-Line streaking method is to get:
A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: what is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of…
A: Answer is option a.) To prevent refraction of light.
Q: 4. Make drawings of your simple stain preparations. Bacillus sp. Staphylococcus sp. Shape Shape…
A: In microbiology the characteristics of the microorganisms is very important. For that purpose…
Q: The technique used in bacteria for endospore staining can also be used to observe fungal spores.…
A: *Endospores staining is used to recognize presence of spore in bacterial cells This needs staining…
Q: Why might gram positive bacteria incorrectly appear to be gram negative after the gram staining…
A: Gram staining is a procedure which is used to identify whether the bacteria being tested in gram…
Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…
A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: the gram stain procedure results provide : Select one: a. all of the above O b. whether they are…
A: Gram staining is a method for differentiating bacterial species into two groups based on physical…
Q: The gram stain is a ______________ stain in which a decolorization step occurs between the…
A: Gram stain is a popular microbiological staining technique for identifying and characterising…
Q: 2. Explain the relationship between bacterial cell wall with gram positive and gram negative in the…
A: Gram staining experiment helps to identify the gram-positive and gram-negative bacteria, and it…
Q: ) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step…
A: On the basis of lipid content and peptidoglycan layer in the cell wall, bacterias are classified…
3. Draw the specimen observed under endospore staining
4. Draw the specimen observed under positive simple staining
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- Define the following terms:a.Simple stainingb.Complex mediumc.Defined (synthetic) mediumd.Parfocale.Negative stainingf. Resolving powerg.Magnifying powerYou can perform a specific staining method to confirm the purity on the culture. Describethis staining method.The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain
- Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore StainIdentify that chemical mordant used in the cold method of Acid-fast staining.The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method
- During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.Differentiate between the “hot method” and “cold method” of acid-fast staining.