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If a broth culture of bacteria, initially containing 37,000 bacteria/ml, were diluted to a 1:1,000 dilution, how many bacteria/ml of the diluted broth would be present on average? Show your solutions
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- You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionYou are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What should be your initial volume from the original culture? Show solution.
- If you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)Compute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values below. Show your solution. Media First Sampling First Sampling First Sampling Second Sampling Second Sampling Second Sampling 10-2 10-3 10-4 10-2 10-3 10-4 VRBA TNTC, TNTC 243, 232 24,22 23, 25 0, 0 0, 0 MRS 32, 40 0, 0 0, 0 TNTC, TNTC TNTC, TNTC 102, 120If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrientbroth, what is the cell density in the new solution?
- Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.What concentration of ethanol is considered optimum for disinfectant? Why not 100%?
- A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solution