The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

The relative polarities of a few common solvents are given below: Solvent name Relative polarity Water 1 Acetic acid 0.65 Acetonitrile 0.48 While using a C-18 hydrocarbon column for protein purification, which of the following will be the best elution solvent mixture? The final polarity of the solvent mixture can be predicted by considering the relative % of each constituent and their respective relative polarity values provide. 1. 90% water and 10% acetic acid II. 90% acetic acid and 10% acetonitrile III. 90% acetonitrile and 10% water IV. 10% acetonitrile and 90% acetic acid

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter10: Fuels, Organic Chemicals, And Polymers

Section: Chapter Questions

Problem 119QRT

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