The sequence of a 29 aa long peptide can be determined from the following data. a) Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val. b) Trypsin digestion, separation of peptides and Edman degradation give sequences for peptide fragments as follows: Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu Ala-Ala-Trp-Gly-Lys Val-Leu-Ser-Pro-Ala-Lys Thr-Asn-Val-Lys

Q: Explain how tandem mass spectroscopy is used to determinethe sequence of a peptide. Once a peptide…

A: The analytical technique that separates gaseous ions based on mass to charge ratio is mass…

Q: A peptide was cleaved into two smaller peptides with cyanogen bromide (CNBr) and into two different…

A: The given peptide sequences are: CNBr 1: Gly-Thr-Lys-Ala-Glu CNBr 2: Ser-Met Tryp 1:…

Q: what would the net charge on the tetra peptide of sequence ala-lys-glu-ser at ph 7.2 what would it…

A: Amino acids are the building blocks of proteins or peptides formed of chiral carbon having four…

Q: Following the IUPAC rules for naming small peptides, assign a name for Leu–Lys–Trp–Met

A: Peptides are short chains of between two and fifty amino acids, linked by peptide bonds. Chains of…

Q: (b) r , The following polypeptide sequence has three alpha helices and two B turns. Based on what we…

A: An amino group and an acid group-containing organic molecules are called Amino acids.…

Q: the peptide (written N-terminal to C-terminal) represented by Ala-Ser-Ala-Tyr the same as the…

A: Ans. Yes, the polypeptide chain of amino acid will be Similarly in structure and function whether it…

Q: A mixture of Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74), Phenylalanine (pl 5.48)…

A: In chromatography, we've got a go with the flow coming out of a column, when we inject a substance…

Q: How is the peptide shown named (give each 3-letter name for the amino acids present)? Whichamino…

A: The peptide is made up of amino acid. The sequence of amino acid in this peptide is N - Val-Phe-Trp-…

Q: Calculate the net charge of the following peptide sequence DAVIRSAXSUEY at pH 7 а. -1 b. -2 с. -3 d.…

A: This peptide contain D, H and E as charged amino acids. all the ionizable groups present in the…

Q: 2.2 Complete hydrolysis of a hexapeptide by 6M HCI at 110°C followed by amino acid analysis…

A: The hydrolysis of the given peptide gives an equal ratio of cysteine, methionine, and glycine. It…

Q: Identify and briefly describe three different PEGylation strategies you can use to PEGylate this…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: Before high performance liquid chromatography (HPLC) methods were devised for the separation and…

A: Peptides are polymers made of multiple amino acid monomeric units. The peptide bonds formed between…

Q: The peptide below NH-CH- HẠN CH- ČH, -NH-CH- NH-CH- NH-CH- OH CH2 CH2 CH2 HN-ç=NH, NH2 CH2 CH HO-CH,…

A: Amino acids are the building blocks of peptides. The net charge on a peptide depends on the sequence…

Q: Indicate all the possible sequences for a tripeptide containing alanine, glutamine, and methionine;…

A: A peptide is the sequence of amino acids that are joined through the formation of peptide bond in…

Q: Write the structure of each of the following peptides. Start with the N-terminal and the COO- on the…

A: Peptides are the sequence of amino acids that are joined through formation of peptide bond. The…

Q: Hydrolysis of dodecapeptide P with the enzyme trypsin affords the following fragments: Glu-Ala-Lys,…

A: Trypsin cleaves the dodecapeptide at the carboxyl side of Lys or Arg. Chymotrypsin cleaves the…

Q: A tridecapeptide yields the following fragments when partially hydrolized. Determine the sequence of…

A: A tridecapeptide is an oligopeptide having thirteen amino acid residues.

Q: Draw the structure of the peptide: Val-His-Leu-Thr-Pro-Val-Glu-Lys-Ser-Ala-Val-Thr

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Write the structure of a tetrapeptide-alanylaspartylvalyltyrosine identify the amino acids in this…

A: A polypeptide is formed when a carboxyl end of one amino acid is linked by the amino-terminal end of…

Q: hymotrypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide…

A: Peptidases are enzymes that act on the peptide bond. Endopeptidases are enzymes that catalyze the…

Q: Remembering that the amino acid side chains projecting from each polypeptide backbone in a β sheet…

A: Amongst secondary structures of polypeptide chains made of amino acids, β-sheets are a more spacious…

Q: At what pH will you be able to separate the amino acids val, his and arg?

A: Amino acids are biomolecules with a central carbon bonded to 4 different groups. 3 of these 4 groups…

Q: What is the Shine–Dalgarno sequence? What role does it play in protein synthesis?

A: The prokaryotic protein synthesis takes place in the ribosomes where the mRNA was translated into…

Q: What is the charge of this peptide at pH 1.5?

A: Amino acids Proteins are the polymers of nitrogenous compounds called amino acids. Each amino acid…

Q: Treatment of a polypeptide by 2-mercaptoethanol yields two polypeptides that have the following…

A: Amino acids are the building blocks of proteins. The amino acid polymer which joins together with…

Q: Define the process of Drawing the Structure of a Peptide from Three-Letter Symbols ?

A: The polypeptide chain is a polymer, which is formed from the incorporation of amino acids. These…

Q: The following steps were performed using enzyme cleavage of a peptide to determine its amino acid…

A: Sequence of amino acids in a peptide are determined by treating the peptide with different agents…

Q: Consider the following peptide sequence: Lys-Leu-Ile-Cys-Lys What is the net charge of this…

A: Physiological pH  Physiological pH, where pH means  power  hydrogen, is the acid base balance in the…

Q: Draw the structure of the tetrapeptide 1. H,N - Lys - Ser-Asp- Ala - COOH Assign IUPAC names to each…

A: Protein or polypeptide is a sequence of amino acids in which the amino-terminal of one amino acid is…

Q: Identify the following non-chiral molecule:  NH2-CH2-COOH (dry form) aspartic acid arginine glycine…

A: Amino acids are small molecules made up of carbon, hydrogen, nitrogen and oxygen in some cases…

Q: The oligopeptide isolated from the brain of an animal has the sequence…

A: Proteins are polypeptide chains formed of monomeric units, amino acids, joined together by a peptide…

Q: Consider the peptides Ser-Glu-Gly-His-Ala and Gly-His-Ala-Glu-Ser. How do these two peptides differ?

A: Introduction: The organic molecules that contain two functional group that is amino and carboxyl are…

Q: Draw the structure of the PTH derivative product you would obtain by Edman degradation of the…

A: Phenyl isothiocynate is the Edman reagent. It reacts with N-terminal amino acid to give phenyl…

Q: what is the mainstream solid phase peptide synthesis ( with detailed mechanisms and intermediates )…

A: Lysine reactive probes are attractive alternatives to cysteine reactive probes when there is no…

Q: You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine…

A: The octapeptide of the fungus has two lysines (K), one amino acid residues of aspartic acid(D),…

Q: Identify which amino acid pairs below will not form R-group interactions necessary for the tertiary…

A: Amino acids are building blocks of proteins, alpha carbon of amino acid contains, carboxyl, amino,…

Q: Treatment of a polypeptide by 2-mercaptoethanol yields two polypeptides that have the following…

A: An amino acid is a natural atom that is comprised of a fundamental amino gathering (−NH2), an acidic…

Q: What is the principal form of arginine at pH 8.0? Approximately what fraction is in this form?

A: Arginine is a basic amino acid which contains an alpha amino group, an alpha carboxylic group and a…

Q: How can we order the peptides to obtain the primary structure of the original protein?

A: Primary structure of protein - These are linear sequence of amino acid that forms the backbone of…

Q: dentify the primary structure of a hexapeptide containing five different amino acids if the…

A: The primary protein structure consists of amino acid sequences. Amino acids are arranged by peptide…

Q: Consider the peptide Asp-Lys-Phe-Glu-Asn-Tyr-Gln-Val-Cys. In a single beaker, you treat this peptide…

A: The amino acids that form peptide chains can be either polar/non-polar, charged/uncharged,…

Q: What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.…

A: The above peptide sequence is made up of five amino acids. First is Lysine, second valine, third…

Q: Based on the following image, what is the N-terminal AA residue, C-terminal AA residue? What is also…

A: With 2,4-Dinitrofluoro benzene forms DNP-Thr so, the N-terminal aminoacid is Thr with Trypsin :…

Q: A mixture of Asparagine (pl 5.41) , Aspartic Acid (pl 2.98), Histidine (pl 7.59), Lysine (pl 9.74)…

A: Cation exchange chromatography separates the molecules based on their net negative charge. Cation…

Q: Draw a complete structural representation for the tetrapeptide Phe-Met-Tyr-Asn (NOTE:use the…

A: Tetrapeptide  is the combination of four amino acids gives linear tetrapeptide, in which three…

Q: Given the following peptides below, what would be more soluble at the indicated pH? 1. (Gly)20 or…

A: Peptides are composed of twenty standard amino acids attached through peptide bonds. These twenty…

Q: Three peptides were obtained from a trypsin digestion of two different polypeptides. indicate the…

A: Three peptides were obtained from trypsin digestion of two different polypeptides.

Q: Which of the peptide sequences below best matches the hydropathy plot shown? L 10 Residue Number 4 2…

A: Hydrophobicity plot analyzes the amount of the hydrophobicity and the hydrophilicity that is present…

Q: Trypsin cleaves a polypeptide backbone at the C-terminal side of Arg or Lys residues, whereas…

A: Polypeptide is formed when amino acids are joined together with peptide bonds. Peptide bond is…