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- In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)Thin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B
- A student spots an unknown sample on a TLC plate. A single spot with an Rf of 0.55 showed up on the plate after developing the sample in hexanes-ethyl acetate 50:50. Does this prove that the unknown is a pure compound?Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answeris hexane or 70:30 hexane: ethyl acetate a better eluent based on their TLC plates and rf value in column chromatography separation of ferrocene-acetylferrocene.
- On the blank TLC provided to the right, please draw where you would expect to see the spots for B-carotene (lane 1), compound A (lane 2) and compound B (lane 3). This is SiO2 plate and the appropriate developing solvent is used.A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the un-known material a pure compound? What can be done to verify the purity of the sample?A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
- The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True FalseAn ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).If a column was run on the sample shown in lane 3, what would be the first compound to elute off of the column? (assume the same solvent and stationary phase are used) Which of the lanes on this TLC plate show pure compounds? Select all that apply.