The spectroscopic data given in the table was generated for two different concentrations of a solution. Concentration (M) Absorbance 0.0147 0.1105 0.1421 0.6015 What is the slope of the line formed by these points? m = What is the y-intercept? b = What is the concentration of a solution with an absorbance of 0.489? concentration: M
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- Complete the information on these tables: wavelength 470 nm conc Um absorbance % transmission 700 0.45 35.58% 500 0.32 47.78% 300 0.19 64.2% 100 0.06 86.23% wavelength 502 nm conc Um absorbance % transmission 700 1.08 8.37% 500 0.77 16.99% 300 0.46 34.57% 100 0.15 70.15% Which mathematical relationship is most useful to relate concentration to the amount of light passing through the solution? (% Transmission, or Absorbance), Why? How does the slope of the absorbance/concentration graph vary with wavelength? Is there an “optimum” wavelength for your KMnO4 sample?A standard curve of absorbance vs. concentration has a linear trendline of y = 0.56x + 0.987. Use this trendline to calculate the concentration of an unknown sample that has an absorbance of 1.245.The nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and then diluted to a total volume of 100.0 mL. The absorbance of this solution determined at a wavelength of 232 nm, was found to be0.3. Several standard solutions were tested under the same conditions and a calibration curve shown below was obtained. Calculate the mass of nickel in mg.
- The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012. You put a solution of unknown concentration into the Spec20 and get an absorbance value of 0.227, which lies within the standards used to make the standard curve. What is the concentration of the unknown solution in mM?A calibration curve is made for the absorbance as a function of concentration of blue dye (in mg/L). The slope of the curve is 0.131; the y-intercept is -0.01869; the correlation is 0.993. If a consumer sample has an absorbance of 0.120 on the same instrument, what is its concentration of blue dye, in mg/L? a. 0.034 b. 0.935 c. 0.993 d. 1.06A relationship between concentration in mg/dL and light absorption in absorbance units is given by the equation: y = 1.2x + 0.28. If concentration is graphed on the x-axis and absorbance is on the y-axis, determine the concentration in mg/dL for a sample with an absorbance value of 0.94
- A plot of Absorbance vs. molarity of Fe(SCN)2+ from part one of the lab gives a straight line with the following equation. y = 51x R2 = 0.998 If a student measures an absorbance value of 0.375 in the second part of the lab, what is the molarity of Fe(SCN)2+ in this solution?The absorbance of various wavelengths of light by a solution were measured. How does absorbance relate to the intensity of light that is transmitted through the solution and measured by the detector?Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.
- Solution. Vol. of A solution (mL) [methyl red], M Absorbance 1 10.00 _1.64 x10^-16_ _0.256_ 2 15.00 _2.45 x10^-16_. _0.373_ 3 20.00 _3.27 x10^-16_. _0.486_ 4 25.00 _4.09 x10^-16_. _0.620_ Plot a graph of the absorbance (y axis) as a function of the concentration of methyl red (x axis). What is the slope of the graph?The concentration of a dilute aspirin solution is 0.000530 M 0.000530 M . Standard solutions of this compound were used to prepare a Beer's law plot which gives a slope of 1550.1 M −1 1550.1 M − 1 . What is the expected absorbance value for the aspirin solution?Spectrophotometric Determination of Nitrate in Seawaters Explain the relationship between absorbance and concentration Explain the principle behind a UV-Vis spectrophotometer (key components and how it works) Why must the absorbance in this experiment be read at a wavelength of 543 nm?