Provide the function of the following in blotting techniques: Nitrocellulose paper  paper weight Probe

Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Various methods have been developed for detecting proteins. Describe how radioisotopes and…

A: Introduction Radioisotopes are the radiolabelled atoms used in tracing the path as they follow from…

Q: how do you purify a protein for single particle cryo-electron microscopy?

A: We would require a protein sample with more than 99% purity in order for it to qualify as a sample…

Q: Describe the Gram staining procedure

A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria.  These…

Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: Transillumination is used clinically for the detection of:

A: Transillumination is an examination that is done with the help of a strong light shining on a…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: FNA

A: The above test is called the COAGULASE test. The coagulase test is a experiment to determine whether…

Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…

A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…

Q: In four steps, describe the simple staining procedure.

A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Describe procedure of RFLP.

A: RFLP (restriction fragment length polymorphism) is an inherited difference in the pattern of…

Q: Molecules are tagged by antibodies in __________ microscopy. a. Confocal b. Differential…

A: Microscopy is defined as that technical field that makes use of microscopes in order to visualize…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: list 4 advantages and 4 limitations of the Gram staining process

A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…

A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: Describe the agar-overlay technique

A: Biological techniques are methods or processes used to study living things. Methods, approaches,…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: Step-by-step smear preparation of Grams stain

A: Gram staining is a laboratory technique that is used to culture bacteria and identifies two kinds of…

Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: Differentiate the “hot method” and “cold method” of acid-fast staining

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…

A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…

Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…

A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…

Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…

A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…

Q: Electro blotting techniques are routinely used in diagnostic microbiology for detection of a…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: Outline in detail the procedure for celloidin embedding .

A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…

A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…

Q: The first step in identifying an unknown bacterium is to __________. culture your unknown on…

A: Bacteria are the minute microorganisms that are unicellular and have a cellular level of…

Q: Differentiate between agarose gel electrophoresis and SDS-PAGE based on composition of gels and dye…

A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…

Q: Write the instructions, reagents, principles and the procedures for the following.  1. Periodic acid…

A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…

Q: What are the advantages and disadvantages of using the Slide Culture technique?

A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…

Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…

A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.

Q: Why is it incorrect to say that the GFP is a “staining” method?

A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

Q: ein isolation an Technique Isolation techniques Centrifugation Salting out Isoelectric precipitation…

A: The purification of a protein is important for determining its structure and characterization of its…

Q: In the bacterial transformation experiment, what is the primary purpose of using an…

A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…

Q: Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and…

A: The gradient-echo (GRE) pulse sequence is also known as gradient recalled echo. The main purpose…

Q: Biuret reagent used in protein assay consists of I. Biuret II. BSA III. Copper (II) sulfate IV.…

A: The biuret test checks for the presence of peptide bonds in the test solution. A peptide bond links…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: 5ml of 1 x 10° cells/ ml cell suspension is required to be prepared for seeding on the cell culture…

A: The hemocytometer is a counting chamber or slide used for the purpose of cell counting. It was…

Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…

A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml