Homework Help is Here – Start Your Trial Now!
Science
Biology
The vector in Figure I below was used in the cloning process. X 2294 Beg 2215 Scal 2177, Gaul 1784. Cfr10 1779 Eco311 1766 Eam11051 1694, bla (Ap") Eco010 274 Aall 2017 Supl 2501 pUC18/19 2686 bp BAP 179 Ehel 235 rep (pMB1) MCS AwN 1217 Sapl 683 AspU1100 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. (ii) Transformation was successful but gene of interest was not present. (iii) Transformation was successful and gene of interest was present.

The vector in Figure I below was used in the cloning process. X 2294 Beg 2215 Scal 2177, Gaul 1784. Cfr10 1779 Eco311 1766 Eam11051 1694, bla (Ap") Eco010 274 Aall 2017 Supl 2501 pUC18/19 2686 bp BAP 179 Ehel 235 rep (pMB1) MCS AwN 1217 Sapl 683 AspU1100 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. (ii) Transformation was successful but gene of interest was not present. (iii) Transformation was successful and gene of interest was present.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
b) -C) a) The vector in Figure I below was used in the cloning process. BAP 179 X 2294 (1) (ii) (iii) Beg 2215 Scal 2177, Gul 1784. Cfr10l 1779 Eco311 1766 Eam11051 1694. Goy) me Eco010 2674 Aarli 2017 Supl 2501 PUC18/19 2686 bp Ehel 235 lacz rep (MB1) MCS AWN 1217 Sapl 3 AfspLU11 806 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. Transformation was successful but gene of interest was not present. Transformation was successful and gene of interest was present. Can the plasmid above in Q1 a) able to produce the protein of interest? Justify your answer. Suggest the vectors from pET system for bacterial protein expression and the host cell of choice. Explain your selection of your host cell.
Transcribed Image Text:b) -C) a) The vector in Figure I below was used in the cloning process. BAP 179 X 2294 (1) (ii) (iii) Beg 2215 Scal 2177, Gul 1784. Cfr10l 1779 Eco311 1766 Eam11051 1694. Goy) me Eco010 2674 Aarli 2017 Supl 2501 PUC18/19 2686 bp Ehel 235 lacz rep (MB1) MCS AWN 1217 Sapl 3 AfspLU11 806 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. Transformation was successful but gene of interest was not present. Transformation was successful and gene of interest was present. Can the plasmid above in Q1 a) able to produce the protein of interest? Justify your answer. Suggest the vectors from pET system for bacterial protein expression and the host cell of choice. Explain your selection of your host cell.
Expert Solution
steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genome annotation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS