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1) What is the purpose in first conducting the inoculation assay prior to treating our SY5Y cells with a chemical for a cytotoxicity assay?

1) What is the purpose in first conducting the inoculation assay prior to treating our SY5Y cells with a chemical for a cytotoxicity assay?

Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
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1) What is the purpose in first conducting the inoculation assay prior to treating our SY5Y cells with a chemical for a cytotoxicity assay? 2) Calculate the volume of cell suspension equivalent to 200,000 cells based on the following hemocytometer data. Cells counted: 257 Squares counted: 3 3) You are conducing calculations for the SRB cell inoculation assay (see protocol). Based on the cell suspension from question 2 above, what total volume of cell suspension will be pipetted into each well of row A in order to set up the serial dilution scheme? Keep in mind, 1/2 this volume (and 1/2 cells!) will be transferred down to row B as the first dilution step. 4) What total volume of cells + complete medium will be pipetted from row A down to row B? How many cells should be suspended within this transfer volume? 5) Given the total volume capacity for each well of a 96-well plate is 350uL, what volume of complete medium will be added to row B prior to transferring cells into row C? 6) How many cells are in row H of inoculation experiment? 7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two sentences or less, explain how this assay works.
Transcribed Image Text:1) What is the purpose in first conducting the inoculation assay prior to treating our SY5Y cells with a chemical for a cytotoxicity assay? 2) Calculate the volume of cell suspension equivalent to 200,000 cells based on the following hemocytometer data. Cells counted: 257 Squares counted: 3 3) You are conducing calculations for the SRB cell inoculation assay (see protocol). Based on the cell suspension from question 2 above, what total volume of cell suspension will be pipetted into each well of row A in order to set up the serial dilution scheme? Keep in mind, 1/2 this volume (and 1/2 cells!) will be transferred down to row B as the first dilution step. 4) What total volume of cells + complete medium will be pipetted from row A down to row B? How many cells should be suspended within this transfer volume? 5) Given the total volume capacity for each well of a 96-well plate is 350uL, what volume of complete medium will be added to row B prior to transferring cells into row C? 6) How many cells are in row H of inoculation experiment? 7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two sentences or less, explain how this assay works.
5) Given the total volume capacity for each well of a 96-well plate is 350uL, what volume of complete medium will be added to row B prior to transferring cells into row C? 6) How many cells are in row H of inoculation experiment? 7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two sentences or less, explain how this assay works. 8) List 3 types of human error that might invalidate or compromise the inoculation assay. 9) What equipment are we using to read our plates? What method is this equipment using to give us data. 10) Why do we run a blank of medium only wells?
Transcribed Image Text:5) Given the total volume capacity for each well of a 96-well plate is 350uL, what volume of complete medium will be added to row B prior to transferring cells into row C? 6) How many cells are in row H of inoculation experiment? 7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two sentences or less, explain how this assay works. 8) List 3 types of human error that might invalidate or compromise the inoculation assay. 9) What equipment are we using to read our plates? What method is this equipment using to give us data. 10) Why do we run a blank of medium only wells?
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