This bacterial component is water soluble polymer, containing a ribol or glycerol residue linked by phosphodiester bonds found in gram positive bacteria: Lipopolysaccharide Endospore Glycocalyx Teichoic acid
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- A cationic microbacteriaI peptide is anaIysed using cation exchange chromatography where the packing materiaI has a negative (-) charge. Choose which buffer system is best used. trishydroxyIammonium ammonia trisaminomethane phosphateDefine the following terms:a. proenzymeb. positive cooperativityc. negative cooperativityd. zymogene. free radicalThe ingredients in the MR/VP Broth are: Peptone (protein) 7g Glucose 5g Phosphate buffer 5g Distilled water 1L Examine the above figure. This figure shows how the pH in the Methyl Red and Voges-Proskauer (MR-VP) broth changes during incubation from time zero to 48 hours with a VP-positive bacteria species. VP-positive bacteria initially produce lactic acid and temporarily lower the pH, but the lowered pH activates the 2,3-butanediol fermentation pathway. Accumulation of its neutral end products such as acetoin and 2,3-butanediol raises the pH by the end of the incubation time. The acetoin that accumulates in the broth also reacts with the reagents added after incubation to produce a red color. If the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical…
- Archaea with cell walls consisting of a thick, homogeneous layer of complex polysaccharides often retain crystal violet dye when stained using the Gram-staining procedure. Suggest a reason for this staining reaction.You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein is currently immobilized. Describe a modification you might make to the buffer that runs across the column to allow the protein to elute from it.The chemical found in gram-positive cell walls and not in gram-negative cell walls reacts with the crystal violet-iodine complex is:
- You are studying the cell walls of gram-negative bacteria. You have isolated the components of their cell walls and run a chemical analysis of what you have isolated. What component listed below would be toxic to eukaryotic cells such as human cells? the peptidoglycan NAG the protein the lignin the cellulose the lipopolysaccharidesA certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table above) into a non-synthetic medium? a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? One that lives in the human stomach? Why are intestinal bacteria able to grow on media containing bile?A patient who is receiving chemotherapy with cisplatin (Platinol) has developed pneumonia. The nurse would be concerned about nephrotoxicity if which type of antibiotic was ordered as treatment for the pneumonia at this time? a )Penicillinb )Sulfa drugc )Fluoroquinoloned )Aminoglycoside
- Which of the following polymer is intracellular? chondroitin sulfate Heparan sulfate Keratan sulfate Heparin sulfateWhich of the following has the highestprotein content per gram?A. AppleB. GroundnutC. SoyabeanD. WheatA 0.10 M NaCl solution is (hypertonic/hypotonic/isotonic) to a 0.1M glucose solution.