This one is "charged" with an aminoacid. The specific sequence in the bottom is called the "Anticodon" which will be a perfect match to a codon in the transcript. Let's focus on this tRNA for Pro:
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- Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also, write out the amino acid sequence of the protein encoded by this message. tRNA: UAC UCU CGA GGC mRNA: protein: How many hydrogen bonds would be present in the DNA segment?The number of codon binding sites in an mRNA-ribosome complex that can be occupied at the same time by activated tRNA molecules is; * A. 2 B. 3 C. 4 D. 5 Which is a characteristics of the normal genetic code? * A. one codon can code for only one amino acid B. one amino acid can have only one codon C. one tRNA molecule can bind to only one codon D. one aminoacyl-tRNA synthetase can react with only one tRNA Which of the following describes the effect of a point mutation on amino acid identity in a protein? * A. The identity of two amino acid must change B. The identity of one amino acid may change C. The identity of one amino acid must change D. The identity of one amino acid never change Which of the following statements is true regarding introns? *…Using the following list of codons describe, using diagrams etc., how information stored in the DNA is translated into a peptide. Be sure to discuss all steps. In other words, use a diagram and give me sequences, transcription and translation steps. Show the sequences of the sense and the other DNA strand, the mRNA and the tRNA’s. UUU -phenylalanine UCU -serine AUG –initiation/methionine CUU -leucine ACU -threonine GUU -valine UAA -Termination
- The tRNA anticodon sequence 3’GAG 5’ is charged with the amino acid leucine. This anticodon may pair with both 5’ CUC 3’ and 5’ CUU 3’. Which of the following is consistent with this phenomenon? 1. The information provided is not accurate since only the 5’ CUC 3’ codon can complementary base pair with the 3’ GAG 5’ anticodon 2. This feature allows for the genetic code to be degenerate 3. This feature allows for the genetic code to be overlapping 4. This feature is commonly associated with tautomeric shifts 5. This feature causes a change in the encoded amino acidThe codon UUU in an mRNA molecule which results in phenylalanine being inserted as the protein is made. Which will be a characteristic of this codon? a. The tRNA molecule that binds to the UUU codon must have an AAA anticodon. Nde ba e2? b. UUU could code for both phenylalanine and alanine during translation. c. The aminoacyl-tRNA synthetase for phenylalanine binds only the UUU codon. d. UUU is probably only one of several codons that code for phenylalanine.Use this mRNA coding sequence as your starting point. This sequence begins with a start codon and ends with a stop codon, so it is only looking at the region of DNA that directly encodes a protein sequence. 5’-AUGCACAAAUUAGAGUACCCCCCAGGAAGGUAG-3’ Make the following mutation in this sequence by changing/adding/removing only one nucleotide. Make the mutation easy to see (a different color, circled, something like that) A missense mutation that is also a transversion
- The codon and anticodon are base-paired together during the process of translation. Which of the following is correct regarding the relationship between anticodon and codon? A. The tRNA anticodon UCU pairs with the AGA mRNA codon. B. The tRNA anticodon is read from the 5’ to the 3’ end. C. A tRNA anticodon can pair with only one mRNA codon. D. The mRNA codon ACG base pairs with the tRNA anticodon TGC.A mutation is found in a tRNA-encoding gene. The wild type (non-mutant) allele (version) produces a tRNA that recognizes the codon GAA, and is charged with the amino acid glutamic acid (Glu). The mutant tRNA is still charged with Glu, but it recognizes the codon UAA. What effect will this have on translation in these cells? How will the proteins produced be different? Speculate: is this mutation more likely to be beneficial or harmful?Yeast have 8 similar tRNA genes with the anticodon 5'-GUA. A researcher mutates one of these genes to change its anticodon to 5'-GUU. a) What codon did the tRNA originally decode? Name the amino acid. b) What codon does the mutated tRNA decode? Name the amino acid.
- The figure below shows a ribosome in the process of translating an mRNA with the sequence: 5’…AUGCCGUAUGCUCUUUAA…3’ a. The right side shows the ribosome with an empty A site aligned with the codon 5’UAU 3’. The next tRNA to occupy the A site on the ribosome will have what anti-codon sequence ( label 5’ and 3’)? Keep in mind the wobble pairing rules and make sure your answer is consistent with the genetic code. Explain your answer. b. Suppose, just as the ribosome started translating the mRNA, the cell suddenly lost all of its alanine tRNAs. Using the figure above as a guide, draw and label the state the ribosome would arrest in.The figure below shows a ribosome in the process of translating an mRNA with the sequence: 5’…AUGCCGUAUGCUCUUUAA…3’ a. The right side shows the ribosome with an empty A site aligned with the codon 5’UAU 3’. The next tRNA to occupy the A site on the ribosome will have what anti-codon sequence ( label 5’ and 3’)? Keep in mind the wobble pairing rules and make sure your answer is consistent with the genetic code. Explain your answer.if the codon for Leucine is 5'-CUG-3'. what is the sequence of the corresponding anticodon on the tRNA-Leu? 5'-CUG-3' 3'-GAC-5' 3'-CUG-5' 5'-GAC-3'