6 When you use the same concentration of phosphoric acid, you get the following one:JI150928_rac_elegy : FXUVUHPDet-4 1:11000900800-7006005004003002001008.10Time (min)Which separation is better? What can cause this behaviour (if all other conditions are the same, save for the acid)?Absorbance (mAU)Uju E82-2.953 min3.986 min5.355 min 3. You are using reverse phase HPLC to separate the cis and trans isomers of the following compound:онWhen you use formic acid to acidify mobile phase, you get the following chromatogram:J150928_rac : FXUVUHPDet-4 1:1600550500450400350300250200150100501012Time (min)Absorbance (mAU)3,098 gimin3,510 min913 MIA-4.220 minBE min5.540min-6.639 min9.331 min

To explain which separation is better.

Answered: Time (min) Which separation is better?…

Time (min) Which separation is better? What can cause this behaviour (if all other conditions are the same, save for the acid)?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.15QAP

See similar textbooks

Related questions

Q: nixture of gaseous reactants is put into a cylinder, where a chemical reaction turns them into gaseo...

Q: Which of these oxides would produce a basic solution in water? A) Na2O b) SiO2 c) SO2 d) Cl...

Q: 44. Select the answer choice that correct identifies if the bonds formed would be nonpolar covalent,...

A: 1) Ionic bonds : bonds that are formed by complete transfer of electrons.Generally formed between a ...

Q: what is the correct significant figure of 560.89 grams of sodium chloride?

A: Given value- > 560.89 gm

Q: CH2 CH2 ċ-OCH3 1. "ОСH; (m) 2. HСI ČH2 CH2-C-OCH3 `CH2

Q: Question 3 Consider the following reaction, which is initially at equilibrium. What happens when HX ...

A: given equilibrium reaction, HX(aq) ⇔ H+(aq) + X-(aq)

Q: Types of volumetric analysis are. * Three. Five. O Four.

A: Titration can be defined as the process of determining the concentration of an analyte by adding sma...

Q: Acetic acid solution 25.3 mL of glacial acetic acid (CH3COOH) dissolved in water to form a 50.0 mL s...

A: Volume of glacial acetic acid = 25.3 mL Volume of solution = 50 mL = 0.05 L

Q: VII. Compound G (C,H120) 11 10 3. HSP-00-009 ppm Chemical Shifts Splitting pattern Peak Area (Normal...

A: Chemical shift splitting pattern Number of protons possible fragment 3.58 quintet/multiplet 1 ...

Q: (c) Explain crystal field splitting in the case of tetrahedral complexes.

A: Crystal field splitting in tetrahedral complexes:

Q: A 12.3 g sample of an alloy of Mg and Al produces 1.66 mol of H2 when added to excess HCI. What perc...

A: Given, Mass of the sample = 12.3 g Mass of H2 gas produced = 1.66 mole What is the percentage of M...

Q: Calculate the standard free-energy change (ΔGo) at 25°C for the reaction H2(g)+O2(g) ⇌ H2O2(l)

Q: Part A Nitric oxide reacts with chlorine gas according to the following reaction: 2 NO (g) + Cl2 (g)...

A: The given chemical reaction is as equilibrium and the reaction is as follows: 2NOg + Cl2g ⇌ 2NOClg A...

Q: 8. The pH at one-half of the equivalence point in a weak acid – strong base titration was determined...

A: Given, pH at one-half of the equivalence point in a weak acid-strong base titration = 4.13 What is...

Q: Explain why an acid-base reaction will only proceed if the products formed are weaker conjugate acid...

A: Answer: In chemistry, reaction is always expected to proceed in the direction where, substances are ...

Q: What does acid do to marble (calcium carbonate)?

A: Calcium carbonate in marble would react with acid to form salt and water. so marble would be eaten (...

Q: A buffer is made using 200.0 mL of 0.100 M (CH3)3N (trimethylamine) and 100.0 mL of 0.200 M (CH3)3NH...

Q: Enhancer 1 A solution of NaCl and water saturated rhen 29.25 g of NaCl has been dissolved in 100 mL ...

A: The concentration of the NaCl solution is = 29.25 g of NaCl dissolved in 100 mL The density of water...

Q: A mixture of gaseous reactants is put into a cylinder, where a chemical reaction turns them into gas...

Q: b. Choose the complete Lewis structure for the following partially completed Lewis structure. F-Br-F...

A: We have find out correct Lewis structure and total valence electrons.

Q: ) Polymers are classified into natural and synthetic polymers. (i) Differentiate between the synthet...

A: Properties of polymers: As per our guideline we have to answer first question only.

Q: The following results were obtained in the replicate determination of the lead content of a blood sa...

A: Relative standard deviation ( S) is 3.76× 10^-3 ppm. coefficient of variation is 0.5 ppm.

Q: One mixes AgNO3 and NaCl. A precipitate forms. That precipitate is: a) AgNa b) NO3Cl c) AgCl...

A: Precipitation Reaction : The chemical reaction occurring in an aqueous solution where two ionic bond...

Q: (b) Calculate the entropy change when two moles of an ideal gas are allowed to expand from a volume ...

A: Given values-> Number of mole=2 mole V1= 1 L V2=10 L T = 27°C =27+273 = 300K

Q: Consider the following reaction: UO₂ (s) + HF (aq) → UF4 (s) + H₂O (1). You mix together 20.0 mL of ...

A: Theoretical yield is the maximum yield of the product that can be obtained in a chemical reaction. ...

Q: This dienol quickly reacts under acidic conditions. Draw the product of this reaction. Include all l...

A: Ans. In given reaction we get two products in that aromatic compound is major because it is most sta...

Q: When the following equation is balanced properly under acidic conditions, what are the coefficients ...

Q: A standard galvanic cell is constructed in which a Sn2+ | Sn half cell acts as the cathode. Which of...

A: Solution 1.Compare the reduction emf of both Sn2+/Sn : E°red = -0.14V Ag+/Ag : E°red = 0.80V We...

Q: Finding mole ratios from chemical formulae This is the chemical formula for methyl tert-butyl ether ...

Q: Use the Henderson-Hasselbach equation to perform the following calculations. The K, of acetic acid i...

A: Molar mass of sodium acetate trihydrate = 136.08 g/mol

Q: What is the pH of a solution that has an H' concentration of 1.0 xX 10 mol/L

Q: Need the mechanical properties of the polymers

A: The polymer is a larger macro-structure that is composed of smaller molecular units repeated over a ...

Q: A 12.3 g sample of an alloy of Mg (35%) and Al (65%) produces H2 when added 25 ml of 1.21 M HCI. Cal...

Q: Which of these base definitions requires OH-. A) Arrhenius only b) Arrhenius and Bronsted c) ...

A: There are various concepts of acids and bases :- Arrhenius concept Brønsted-Lowry concept Lewis ...

Q: Give the formula for the following lonic compounds. a. potassium sulfate b. magnesium carbonate C. a...

A: The chemical Formula is given by Naming metal first followed by non metal.

Q: Answer the questions regarding the tripeptide shown. HN-CH- -C- -N-CH-C- -N-CH-C-0" CH CH H,C CHOH C...

A: Amino acid are compound which have amine and COOH group Here We are required to find the N terminal ...

Q: Which one hardness type that remain after boiling process. O Temporary hardness. O Permanent hardnes...

A: 2nd option is true that is permanent hardness

Q: Sulfur dioxide and oxygen react to form sulfur trioxide, like this: 2 SO,(9)+O2(g) –→2 SO3(g) The re...

A: Recall the given reaction, 2 SO2 (g) + O2 (g) 2 SO3 (g) is an exothermic...

Q: Describe the following gel matrices used in gel filtration chromatography and discuss the types of s...

Q: Write the corect Lewis structure and assign a formal charge to each atom in the fulminate ion, (CNO)...

A: Given :- formula of fulminate ion = (CNO)- To write :- correct lewis structure of fulminate ion and...

Q: 18 ic weights are based on the Atomic Elements 1995 publishedby IUPAC Не 13 14 15 16 17 4.003 6. 10 ...

Q: 2 3. NEXT > Fill in the ICE table with the appropriate value for each involved species to determine ...

Q: What is the change in volume for a system under a constant 2 atm pressure that releases 807 J of hea...

A: given :- heat released = 807 J constant pressure = 2 atm change in internal energy = -300.5 J 1 ...

Q: A mixture of He, Ne, and Ar has a pressure of 28.2 bar at 28.0 °C. The partial pressure of He is 2.7...

A: given :- partial pressure of He = 2.74 bar partial pressure of Ar = 1.15 bar total pressure of mix...

Q: Assign IUPAC names to the following ketones: || a. CH,CH,CH,CH,CH, -C-CH, || b. CH,CHCH -С-СНСH, CH,...

Q: statement. (e) Why methyl chloride absorbs at lower wavelength than methyl iodide?

A: Wavelength of Methyl chloride is approx. 172-175 nm and Wavelength of methyl iodide approximate 25...

Q: Types of volumetric analysis are. * O Three. O Five. O Four.

A: volumetric analysis : This method is also known as Titration.

Q: #lockdown 问题11 Which of the following indicate a beta configuration at the anomeric carbon? 559 CH,O...

A: Anomeric are cyclic monosaccharides that are epimer and differ in confirmation at C1 carbon. alpha-...

Q: The Lewis structure of the molecule, NH,O*, contains a) b) c) d) 1 double bond and 4 single bonds 5 ...

A: The Lewis structure of a molecule describes the distribution of valence shell electrons around each ...

Q: A mixture of gaseous reactants is put into a cylinder, where a chemical reaction turns them into gas...

A: Given data :- Amount of energy absorbed = 393 KJ Work done by the piston on the system = 194 KJ

Question

When you use the same concentration of phosphoric acid, you get the following one:
JI150928_rac_elegy : FXUVUHPDet-4 1:1
1000
900
800-
700
600
500
400
300
200
100
8.
10
Time (min)
Which separation is better? What can cause this behaviour (if all other conditions are the same, save for the acid)?
Absorbance (mAU)
Uju E82
-2.953 min
3.986 min
5.355 min

3. You are using reverse phase HPLC to separate the cis and trans isomers of the following compound:
он
When you use formic acid to acidify mobile phase, you get the following chromatogram:
J150928_rac : FXUVUHPDet-4 1:1
600
550
500
450
400
350
300
250
200
150
100
50
10
12
Time (min)
Absorbance (mAU)
3,098 gimin
3,510 min
913 MIA
-4.220 min
BE min
5.540min
-6.639 min
9.331 min

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Quality Assurance and Calibration Methods

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67 Group of answer choices II > IV > III > I I > III > IV > II I > II > III > IV IV > II > III > I
1. What is the retention time of molecule 9?2. What happens to the peak area if the sample amount is doubled?3. If run time is stopped at 5.5 mins, will molecules 8 & 6 be separated and quantified?4. If the flow rate was doubled, will 8&6 be separated and quantified?5. Does a single peak at time T in the chromatogram indicate only one compound is eluted?
The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
A protein consists of two types of peptide chains (A and B) with an unknown stoichiometry (AxBy). When you ran this protein directly on reversed phase-HPLC with UV monitor set at 280 nm, two peaks were resolved. Mass spec determined that the peaks represented Chain A and Chain B, respectively. The peak area is 500,000 for Peak A (Chain A) and 100,000 for Peak B (Chain B). The molecular masses of Chain A and Chain B are 25,000 and 5000, respectively. The extinction coefficients for Chain A and Chain B are 1 mL/mg.cm and 0.5 mL/mg.cm, respectively. Please calculate x/y.
In what order would the following compounds be eluted on a silica gel TLC plate if they are spotted on the same plate and developed in a solvent mixture of 10:1 hexanes:ethyl acetate A. 1 has the highest Rf value, then 2 then 4 then 3 b. 4 has the highest Rf value, then 1 then 3 then 2 c. 3 has the highest Rf value, then 2 then 1 then 4 d. 2 has the highest Rf value, then 3 then 1 then 4
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
From this chromatogram, which of the following compounds is the MOST polar, 14, 10, or, 3?
You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape? If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?
Identify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…
How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solvent
1. Describe the different types of analytes which can be analyzed using the different methods of optical analyses. 2. Why is it that absorbance readings be done at lambda max?
You and another student were each given an unknown compound. Both samples contained colorless material. You each used the same brand of commercially prepared TLC plate and developed the plates using the same solvent. Each of you obtained a single spot of Rf = 0.75. Were the two samples necessarily the same substance? How could you prove unambiguously that they were ily identical using TLC?