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In determing Rh group, do you consider the control tube for reading when it shows an agglutination reaction? Kindly explain
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- If detected in antibody screen testing, which of the following antibodies is NOT considered clinically significant in prenatal patients? Please select the single best answer Anti-M Anti-N Anti-Leb Anti-FyaYou just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?A thyroid peroxidase antibody test was performed on a 45 year old man. The dilution sequence was 25 microliters serum to 100 microliters of diluent in tube 1. Then 25 microliters from tube 1 was added to 25 microliters of diluent in tube 2. Then 25 microliters from tube 2 was added to 50 microliters of diluent in tube 3. What is the dilution factor of tube 1. What is the dilution factor in just tube 2. What is the dilution factor in just tube 3. What is the overall dilution factor in tube 3.
- Briefly explain the significance of RH factor. Why mother with Rh negative blood should receive RhoGAM shot? What type of antibodies would be produced in this case? Why there is no universal donor anymore? What type of blood you can transfuse if patient has type A- blood?In agglutination reactions, the antigen is a……… in precipitation reactions, the antigen is a……………RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…
- RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody reactivity is enhanced by acidification?A thyroid peroxidase antibody test was performed on a 45 year old man. The dilution sequence was 25 microliters serum to 100 microliters of diluent in tube 1. Then 25 microliters from tube 1 was added to 25 microliters of diluent in tube 2. Then 25 microliters from tube 2 was added to 50 microliters of diluent in tube 3. A.What is the dilution factor of tube 1? B. What is the dilution factor in just tube 2? C.What is the dilution factor in just tube 3? D.What is the overall dilution factor in tube 3?(leave answer as fraction)