Tom streak plated an Unknown bacterium on Monday. On Wednesday, her plate showed a continuous streak of growth in two colors, but no distinct single colonies. List two possible reasons for these observations and why they may have affected the streak.
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- Describe the difference between the appearance of surface and subsurface colonies in a pour plate. If this is the same bacterial species, why do these differences in colonial growth occur?I tested organism growth on the MSA plate for strep. pyongenes, strep. pneumonia, s. aureus, and straph. epidermidis and my results were that streptococci organism did not grow or change color and the s.aureus changed color (yellow) and s.epi there was no change in color but they both showed growth. 1. If an unknown gram-positive bacterium was given, what would it mean if it grew on MSA? Name the organisms used above My answer: that it would be either s.aureus or s. epidermis because they are selective for salt-tolerant bacteria and can grow on mannitol 2. what would it mean if it both grew on MSA and turned yellow? Can you add to my answer or correct it. Thank you!In not more than three pages, describe the bacteria cell wall, hence differentiate between the gram positive and the gram negative bacteria with not less than five examples and the specific disease each of them causes.
- • Which bacterium produced a yellow halo, and how do you interpret this result? • If you grew Staphylococcus epidermidis on MSA, what result would you expect?E.coli was incubated with aeration in a nutrient medium containing two carbon sources provided one at a time, and the following growth curve was made from this culture. a. Explain what happened at the time marked x. b. Which substrate provided “better” growth conditions for the bacteria? How can you tell?After the original specimen has been plated and a reported gram-negative rod was seen on the initial gram stain, what is your initial assumption of the organism causing this disease?
- what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?You are doing water analysis. After 24 hours, your pour-plate reveals the presence of Proteus-looking colonies. What is the explanation why you need to eliminate these plates from your experimental data?Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to be in fading color and the shape of the specimen seems to be diffused. How are you going to troubleshoot the focusing of the specimen for you to identify clearly the bacteria?
- Did each handwashing treatment result in similar types of organisms growing on the plates? (look at the colonies and gram types) Explain why or why not?A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found ? What errors could have contributed to the variable result?