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- QUESTION: Microbes from a blood culture inoculated with blood drawn from a catheter were determined by the appearance of growth on Mannitol Salts Agar and through other tests to be Staphylococcus epidermidis. It can be concluded that: a. a bloodstream infection caused by S. epidermidis is occurring in the patient from whom the blood was drawn b. The bacteria fermented mannitol, changing the color of the growth medium from pink to yellow. c S. epidermidis is present in the sample as a normal flora contaminant d. That in order to determine if a bloodstream infection is present, these results must be compared to a blood culture of a sample drawn from a peripheral veinGuide Questions: Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used?Topic: Multiple Tube Fermentation Questions: 1. What protocol should you follow if gas formation is less than 10%? a. Why not test for pathogens directly instead of usimg indicator organism such as coliform bacteria? b. Can you use the MPN method to test for the presence of coliforms in food? Why or why not? c. Describe another method of determining the presence of coliforms in a water sample.
- All questions a) Name structures P, Q and R. b) Name substance S . c) What is the enzyme encoded by gene I. Give its function. d) What will happen if substance S is absent in the medium?Q5 Endospores are very resistant to heat, drying, radiation and chemicals.A) TrueB) FalseQuestion 7 Microbiology BIOL 2420 What does the line at the end of the arrow represent? Question 7 options: a) therapeutic index of the antibiotic b) minimum inhibitory concentration c) end of diffusion of the antibiotic d) more than one of these
- need assistance with microbiology questions 1. The agar in nutrient agar microbiological growth medium serves as a------. A. carbohydrate source B. solidifying agent C. medium supplement D. protein source 2. A mycelium is _______. A. mutualistic relationship between a fungus and a plant B. a reproductive structure in fungi C. a mass of connected fungal hypae D. a partition that divided fungal hyphae into cell like compartments 3. Cleaning the wound with Hydrogen peroxide is an example of A. bacteriostasis B. sterilization C. antisepis D. disinfection 4. You are asked to prepare 5% bleach solution to be used on certain surfaces at work. The only Bleach solution you have available is 10%. In order to make the desired dilution what is the volume of 10% bleach you will use to make 1000 ml of bleach solution. A. Take 150 ml of 10% bleach and make the volume up to 1000 ml B. Take 50 ml of 10% bleach and make the volume up to 1000 ml C. Take 5 ml of 10% bleach and make the volume up to 1000…General Electrophoresis Questions: 1. What makes macromolecules move through the gel in electrophoresis?2. What determines the speed at which macromolecules move through the gel in electrophoresis? In a single gel, why do some move faster than others?3. Why do we use different procedures for DNA and protein electrophoresis?Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?
- Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…Problem 1: At a total magnification of 100x, a student measures 16.4 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 100x? Problem 2: At a total magnification of 400x, a student measures 4.1 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 400x? Problem 3: Notice the pattern in the above 2 problems. What do you think the distance in micrometers per ocular unit at 1000x would be?question- Can lactobacillus bacteria (found in human digestive and urinary tracts) be considered an extremophiles?